Recombinant Mouse Neurofilament medium polypeptide (Nefm), partial

中文名稱：

小鼠Nefm重組蛋白
貨號：

CSB-YP015691MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Nefm重組蛋白
貨號：

CSB-EP015691MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Nefm重組蛋白
貨號：

CSB-EP015691MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Nefm重組蛋白
貨號：

CSB-BP015691MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Nefm重組蛋白
貨號：

CSB-MP015691MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nefm
Uniprot No.：

P08553
別名：

Nefm; Nef3; Nfm; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
基因功能參考文獻：
1. Gene deletion studies demonstrated for the first time that lack of NFM leads to changes of D1R-mediated long-term potentiation and D1R-mediated behavior PMID: 25869803
2. Data conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons. PMID: 23028520
3. Computational modeling predicted that an expanded NF-M C terminus extended farther from the neurofilament core independent of lysine-serine-proline (KSP) phosphorylation. PMID: 22553027
4. results obtained in mouse model for leprous neuropathy indicate biochemical alterations in neurofilaments, i.e., hyperphosphorylation of NF-H and NF-M PMID: 20306637
5. Data show that the tail domain of neurofilament subunit NF-M, with seven KSP motifs, is an essential target for the myelination-dependent outside-in signaling cascade that determines axonal caliber and conduction velocity of motor axons. PMID: 14662745
6. results demonstrate NF-M tail-mediated interactions of neurofilaments, independent of NF transport rate, are critical determinants of the size and cytoskeletal architecture of axons and are mediated in part by the highly phosphorylated tail domain of NF-M PMID: 14662746
7. NF-M regulates the association of NFs with kinesin. PMID: 16246456
8. Alpha-internexin coassembles with all 3 neurofilament proteins into a single network of filaments in quadruple-transfected cells. Deleting NF-M alone or together with NF-H in mice reduces alpha-internexin transport & content in axons throughout the CNS. PMID: 17005864
9. data indicate that a blockade of NMDA receptors during development of cortical neurons increases the phosphorylation state and the solubility of NF-M, thereby favoring neurite outgrowth PMID: 18412220
10. Phosphorylation of NF-M lysine-serine-proline (KSP) repeats is not an essential component for the acquisition of normal axonal caliber mediated by myelin-dependent outside-in signaling. PMID: 19193875
11. RE1 silencing transcription factor (REST) plays a contributory role in the neuron-specific expression of the alpha-internexin, NF-H and NF-M genes PMID: 19457133
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亞細胞定位：

Cytoplasm, cytoskeleton. Cell projection, axon.
蛋白家族：

Intermediate filament family
組織特異性：

Expressed in the sciatic nerve (at protein level).
數據庫鏈接：

KEGG: mmu:18040

STRING: 10090.ENSMUSP00000022638

UniGene: Mm.242832