Recombinant Mouse DNA replication licensing factor MCM2 (Mcm2), partial

中文名稱：

小鼠Mcm2重組蛋白
貨號：

CSB-YP013590MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Mcm2重組蛋白
貨號：

CSB-EP013590MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Mcm2重組蛋白
貨號：

CSB-EP013590MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Mcm2重組蛋白
貨號：

CSB-BP013590MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Mcm2重組蛋白
貨號：

CSB-MP013590MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mcm2
Uniprot No.：

P97310
別名：

Mcm2; Bm28; Cdcl1; Kiaa0030; Mcmd2; DNA replication licensing factor MCM2; EC 3.6.4.12; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
基因功能參考文獻：
1. Segregation of parental histones to the leading strand increased markedly in cells with histone-binding mutations in MCM2, part of the replicative helicase, exacerbating histone posttranslational modifications sister chromatid asymmetry. PMID: 30115746
2. MCM2 is dynamically expressed in both proliferative and differentiated stromal cells during mouse periimplantation uterus. expression of Mcm2 is induced by progesterone action in the mouse uterine stroma. siRNA-mediated silencing of MCM2 arrests the cell cycle at G1-S transition during stromal cell proliferation. downregulation of Mcm2 could also compromise stromal cell differentiation. PMID: 26910396
3. MCM2 deficiency reduces DNA replication initiation in gene-rich regions of the genome. PMID: 25762552
4. these data demonstrate that active MCM2-7 repression is a physiologically important mechanism for RS-induced cell cycle arrest and genome maintenance on an organismal level. PMID: 26765334
5. Hematopoietic cells originally express higher levels MCM 2 protein and undergo more frequent apoptosis following doxorubicin-induced DNA-damage in the presence of the FLV envelope protein gp70. PMID: 22768239
6. Mcm2 deficiency results in short deletions allowing high resolution identification of genes contributing to lymphoblastic lymphoma. PMID: 22158038
7. Accumulation and dynamics of Mcm2 are described during oogenesis and the first embryonic cell cycle. PMID: 17554680
8. gp70 enhances cellular DNA-damage-induced signaling in association with host-specific cellular proteins including acinus and MCM2 resulting in the activation of DNA-PK to phosphorylate P53. PMID: 19058849
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

MCM family
數據庫鏈接：

KEGG: mmu:17216

STRING: 10090.ENSMUSP00000061923

UniGene: Mm.16711