Recombinant Mouse Apolipoprotein M (Apom)

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品名簡稱：

Recombinant Mouse Apom protein
貨號：

CSB-EP001947MO
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Apom
Uniprot No.：

Q9Z1R3
別名：

(Apo-M)(ApoM)
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

27.2 kDa
表達區域：

1-190aa
氨基酸序列

MFHQVWAALLSLYGLLFNSMNQCPEHSQLTALGMDDTETPEPHLGLWYFIAGAASTTEELATFDPVDNIVFNMAAGSAPRQLQLRATIRTKSGVCVPRKWTYRLTEGKGNMELRTEGRPDMKTDLFSSSCPGGIMLKETGQGYQRFLLYNRSPHPPEKCVEEFQSLTSCLDFKAFLVTPRNQEACPLSSK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
基因功能參考文獻：
1. apoM-S1P-S1PR1 signaling might underlie the pathogenesis of ALI and apoM could have physiological benefits to alleviate LPS-induced ALI. PMID: 29260347
2. These S1P-induced enhancements in growth factors and chemotactic cytokines in retinal pigment epithelium cells were significantly inhibited by ApoM treatment. Additionally, in vivo experiments using a laser-induced choroidal neovascularization (CNV) murine model demonstrated that intravitreal ApoM injection significantly reduced the progression of CNV formation. PMID: 29301231
3. This study highlights the complexity of Apom/S1P in atherosclerosis and challenges the notion that the Apom/S1P complex is anti-atherogenic, at least in Apoe-deficient mice. PMID: 28866363
4. This suggests that the autophagy dysfunction caused by the deficiency of ApoM is an important factor in hepatic steatosis (triglyceride accumulation). ApoM plays a key role in normal autophagy activity in the liver and thereby further regulates the metabolism of liver lipids, particularly triglycerides. PMID: 29288662
5. HDL facilitates S1P efflux from erythrocytes by both apoM-dependent and apoM-independent mechanisms. Moreover, apoM facilitates tubular reabsorption of S1P from the urine, however, with no impact on S1P plasma concentrations. PMID: 24950692
6. The study suggests that vascular leakage of albumin-sized particles in ApoM deficiency is S1P- and S1P1-dependent and this dependency exacerbates the response to inflammatory stimuli. PMID: 26956418
7. apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the T-lymphocytes subgroups in murine spleen PMID: 26543853
8. Upon immune stimulation, Apom(-/-) mice developed more severe experimental autoimmune encephalomyelitis, characterized by increased lymphocytes in the central nervous system and breakdown of the blood-brain barrier PMID: 26053123
9. LDL receptor and ApoE have roles in the clearance of ApoM-associated sphingosine 1-phosphate PMID: 25505264
10. ApoM augmented insulin secretion by maintaining the S1P concentration under both in vivo and in vitro conditions. PMID: 24814049
11. The present data indicate that the plasma apo-M levels modulate the ability of plasma to mobilize cellular cholesterol, whereas apo-M has no major effect on the excretion of cholesterol into feces. PMID: 24046869
12. ApoM can bind oxidized phospholipids, increasing the antioxidant effect of HDL. PMID: 22204862
13. Results show that apoM, by delivering S1P to the S1P(1) receptor on endothelial cells, is a vasculoprotective constituent of HDL. PMID: 21606363
14. After refolding from inclusion bodies, the crystal structure of apoM (reported here at 2.5 A resolution) displays a novel yet unprecedented seven-stranded beta-barrel structure. PMID: 20932978
15. apoM mainly associates with HDL in normal mice but also with the pathologically increased lipoprotein fraction in genetically modified mice; decreased apoM levels in apoA-I-deficient mice suggest a connection between apoM and apoA-I metabolism. PMID: 15102887
16. ApoM transcripts were detectable in mouse embryos from day 7.5 to day 18.5 PMID: 15147633
17. in both liver and kidney, expression of apoM was significantly lower in leptin deficient ob/ob mice and in leptin-receptor deficient db/db mice than in control mice PMID: 15358114
18. Overexpression of apoM in Ldlr(-/-) mice protected against atherosclerosis when the mice were challenged with a cholesterol-enriched diet. PMID: 15793583
19. Megalin-mediated endocytosis in kidney proximal tubules prevents apoM excretion in the urine. PMID: 16099815
20. Based on these results, we suggest that insulin regulates apoM synthesis in vivo and, therefore, that the reduction of apoM expression is a general phenomenon in diabetes models. PMID: 16516154
21. In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. PMID: 18006500
22. Apolipoprotein M is a negative acute response protein that decreases during infection and inflammation PMID: 18054359
23. Foxa2 activity increases plasma high density lipoprotein levels by regulating apolipoprotein M PMID: 18381283
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亞細胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
組織特異性：

Expressed by the liver; secreted in plasma.
數據庫鏈接：

KEGG: mmu:55938

STRING: 10090.ENSMUSP00000025249

UniGene: Mm.2161