Recombinant Human Triple functional domain protein (TRIO), partial

中文名稱：

人TRIO重組蛋白
貨號：

CSB-YP024521HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人TRIO重組蛋白
貨號：

CSB-EP024521HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人TRIO重組蛋白
貨號：

CSB-EP024521HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人TRIO重組蛋白
貨號：

CSB-BP024521HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人TRIO重組蛋白
貨號：

CSB-MP024521HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

TRIO
Uniprot No.：

O75962
別名：

ARHGEF23; MEBAS; MRD44; PTPRF-interacting protein; tgat; trio; Trio Rho guanine nucleotide exchange factor; TRIO_HUMAN; Triple functional domain (PTPRF interacting); Triple functional domain protein
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases. Involved in coordinating actin remodeling, which is necessary for cell migration and growth. Plays a key role in the regulation of neurite outgrowth and lamellipodia formation. In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses. May act as a regulator of adipogenesis.
基因功能參考文獻：
1. Results revealed that TRIO high expression levels in cervical cancer were correlated with lymph node metastasis. Its inhibition significantly decreased the migration and invasion abilities of cervical cancer cells through the RhoA/ROCK signaling pathway. PMID: 29207149
2. Trio plays a role in activating Rac1 in podocytes both in basal conditions and when stimulated with TGFbeta1. We speculate that Trio may have dual roles; it may play important roles in podocyte development but it may also regulate pathological Rac1 hyperactivation in the context of TGFbeta1-mediated podocyte injury and glomerulosclerosis. PMID: 29415466
3. Genetic damage to both TGEF domains altered TRIO catalytic activity, decreasing TGEF1 activity and increasing TGEF2 activity. PMID: 28973398
4. A recurrent rearrangement involving TRIO with various partners was identified in 5.1% of non-translocation-related sarcoma cases. TRIO translocations are either intrachromosomal with TERT or interchromosomal with LINC01504 or ZNF558. All translocations led to a truncated TRIO protein either directly or indirectly by alternative splicing. TRIO rearrangement is associated with a modified transcriptomic program. PMID: 27528700
5. Study identifies a large cluster of de novo mutations in the GEF1 domain of Trio in whole-exome sequencing data from individuals with autism spectrum disorder. PMID: 28928363
6. The findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis. PMID: 27907191
7. TRIO loss of function is associated with mild intellectual disability and affects dendritic branching and synapse function. PMID: 26721934
8. our data show the importance of spatio-temporal regulation of the actin cytoskeleton through Trio and Rac1 at VE-cadherin-based cell-cell junctions in the maintenance of the endothelial barrier. PMID: 26116572
9. Aberrant expression of TRIO might play an important role in hepatocellular carcinoma (HCC) through promoting cell proliferation and invasion, and TRIO may be a novel therapeutic target for the treatment of HCC PMID: 25851347
10. Strong statistical evidence for a causal role of TRIO in neurodevelopmental and neuropsychiatric disorders. PMID: 26235986
11. TRIO controls Rac1 activation in dividing cells which counteracts MgcRacGAP function in cytokinesis. PMID: 25355950
12. The +TIP Navigator 1 (NAV1) is important for neurite outgrowth and interacts and colocalizes with TRIO, a Rho guanine nucleotide exchange factor that enables neurite outgrowth by activating the Rho GTPases Rac1 and RhoG. PMID: 25065758
13. TRIO is an EB1 dependent MT plus end tracking protein, and forms a complex with NAV1 at growing MT ends, which regulates TRIO-mediated Rac1 activation and neurite outgrowth. PMID: 25065758
14. Describe here an invadopodia disassembly model, where a signalling axis involving TrioGEF, Rac1, Pak1, and phosphorylation of cortactin, causes invadopodia dissolution. PMID: 24859002
15. We conclude that Trio promotes leukocyte transendothelial migration by inducing endothelial docking structure formation in a filamin-dependent manner through the activation of Rac1 and RhoG. PMID: 22696684
16. Trio, is essential for activating Rho- and Rac-regulated signaling pathways acting on JNK and p38. PMID: 23177739
17. GEF Trio is responsible for lamellipodia formation through its N-terminal DH-PH domain in a Rac1-dependent manner during fibronectin-mediated spreading and migration. PMID: 22238672
18. present the x-ray structure refined to 1.7-A resolution and effect on signaling pathway by PI 3-kinase PMID: 15199069
19. TRIO amplification is associated with invasive tumor growth and rapid tumor cell proliferation in bladder neoplasms. PMID: 15215162
20. Expression of Tgat in NIH3T3 resulted in the loss of contact inhibition, increase of saturation density, anchorage-independent growth, tumorigenicity in nude mice, and increased invasiveness. PMID: 15308664
21. We have identified neuronal specific isoforms of Trio which could be essential for Trio function in neuronal morphology. PMID: 16033331
22. TRIO represents a candidate target of 5p amplifications in soft tissue sarcomas and might play a crucial role during the progression of this disease. PMID: 16752383
23. Results support a general role for DH-associated PH domains of TRIO in engaging Rho GTPases directly for efficient guanine nucleotide exchange. PMID: 17391702
24. Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain PMID: 17606614
25. Data show that Trio, Ect2, and Vav3 are expressed at higher levels in glioblastoma versus low-grade glioma, and are involved in tumor cell migration and invasion. PMID: 19008376
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相關疾病：

Mental retardation, autosomal dominant 44 (MRD44)
亞細胞定位：

Cytoplasm. Cell projection.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family
組織特異性：

Widely expressed, with highest levels in heart, skeletal muscle, and brain.
數據庫鏈接：

HGNC: 12303

OMIM: 601893

KEGG: hsa:7204

STRING: 9606.ENSP00000339299

UniGene: Hs.130031