Recombinant Human Transcription factor E2F3 (E2F3)

中文名稱：

Recombinant Human Transcription factor E2F3 (E2F3)
品名簡稱：

Recombinant Human E2F3 protein
貨號：

CSB-EP007342HUc7
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

E2F3
Uniprot No.：

O00716
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

56.1 kDa
表達區域：

1-465aa
氨基酸序列

MRKGIQPALEQYLVTAGGGEGAAVVAAAAAASMDKRALLASPGFAAAAAAAAAPGAYIQILTTNTSTTSCSSSLQSGAVAAGPLLPSAPGAEQTAGSLLYTTPHGPSSRAGLLQQPPALGRGGSGGGGGPPAKRRLELGESGHQYLSDGLKTPKGKGRAALRSPDSPKTPKSPSEKTRYDTSLGLLTKKFIQLLSQSPDGVLDLNKAAEVLKVQKRRIYDITNVLEGIHLIKKKSKNNVQWMGCSLSEDGGMLAQCQGLSKEVTELSQEEKKLDELIQSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKAPPETRLEVPDSIESLQIHLASTQGPIEVYLCPEETETHSPMKTNNQDHNGNIPKPASKDLASTNSGHSDCSVSMGNLSPLASPANLLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEKLPLVEDFMCS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters.
基因功能參考文獻：
1. Results suggest that E2F3b isoform functions as an important promoter for cell proliferation and plays important roles in transcriptional regulation in HepG2 liver cancer cells. PMID: 29135049
2. Mechanistic investigations suggested that hsa_circ_0008039 served as a competing endogenous RNA (ceRNA) of miR-432-5p. Subsequently, E2F3 was identified as the functional target of miR-432-5p and overexpression of hsa_circ_0008039 elevated E2F3 expression in breast cancer. PMID: 29807010
3. miR433 may inhibit the progression of nonsmallcell lung cancer, at least in part, by targeting E2F3. The present study indicated that miR433 may be investigated as an innovative candidate target for the therapy of patients with this fatal disease. PMID: 29767254
4. our findings indicated that miR-152 reduced osteosarcoma growth and invasion by targeting E2F3 PMID: 28810933
5. these results revealed that miR-363 is involved in hepatocellular carcinoma (HCC)growth and invasion and functions as a tumor suppressor by negatively regulating E2F3. PMID: 29039555
6. E2F3 and ephrin A3 are putative targets of miR-210, and their protein expression was up-regulated in the angiosarcoma cells. PMID: 28739548
7. The increased expression of miR-503-5p significantly reduced the expressions of E2F transcription factor 3 (E2F3) mRNA and retinoblastoma protein (Rb)/E2F signaling pathway mRNA in bladder cancer cells. PMID: 29169421
8. Results of real-time PCR and Western blot showed that the depletion of E2F3a upregulated the expression levels of cell apoptosis-related proteins and downregulated migration-related proteins. Conversely, E2F3a overexpression downregulated the expression levels of cell apoptosis-related proteins and upregulated migration-related proteins. PMID: 28337965
9. lncRNA H19 may function as the sponge of miR-106a-5p to up-regulate E2F3 expression, and consequently promote the glucose metabolism and growth of melanoma. PMID: 29350287
10. Blood E2F3 mRNA is a sensitive diagnostic marker in lung cancer; moreover, it is a promising prognostic marker capable of efficiently discriminating early from late stages of the disease PMID: 28269748
11. miR128-1 could function as a tumor suppressor in glioblastoma multiforme by negatively regulating tumor cell proliferation, invasion and self-renewal through direct targeting BMI1 and E2F3. PMID: 27705931
12. NEAT1 modulated the expression of E2F3 and MiR377 and promoted non small cell lung cancer progression. PMID: 27351135
13. High expression level of E2F3 is associated with lung adenocarcinoma. PMID: 26942465
14. the current study demonstrated that miR34a suppressed the proliferation and induced apoptosis of SRA01/04 cells by downregulating E2F3. PMID: 27840975
15. we investigated how Rb is integrated to control bladder cancer progression through E2F3 and p53 regulation. The results exhibit that Rb expression is lower in patients with bladder tumor, while E2F3 level is high. Rb knockdown enhanced bladder tumor cell proliferation and migration, aggravated with p53 silence. PMID: 27922689
16. By downregulating E2F3 (an miR-497 target gene) mRNA and protein. PMID: 27430325
17. miR-874 expression was significantly decreased in OS cell lines and tissues, and its expression was correlated with TNM stage, tumor size, and lymph node metastasis; miR-874 inhibited cell proliferation, migration, and invasion and induced cell apoptosis of OS cells partially by targeting E2F3. PMID: 26631042
18. miR-503 inhibits cell proliferation and induces apoptosis by directly targeting E2F3 in colorectal cancer cells. PMID: 26722476
19. E2F3 silencing decreases Her2-positive mammary tumor growth by reducing percentage of cells undergoing mitosis. PMID: 26512919
20. mirn424 has a role in inhibiting Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma PMID: 26315541
21. miR-145 expression was lower in tumors compared with matched normal samples and correlated with increased the E2F3 transcription factor protein staining. PMID: 25762621
22. miR-200c appears to control the epithelial-to-mesenchymal transition process through BMI-1 in bladder cancer cells, and it inhibits their proliferation through down-regulating E2F3. PMID: 25367080
23. In gastric cancer miR-141 could be interacting with MEG3 and targeting E2F3 to inhibit cell proliferation. PMID: 26233544
24. our study demonstrated that miR-34c plays a role of tumor suppressor in HEC-1-B cells, and E2F3 protein may be a target of miR-34c. PMID: 25846116
25. MiR-377 is an important negative regulator of E2F and MAP3K7/NF-kB signaling pathway in melanoma cells. PMID: 25889255
26. MiR-203 sensitizes glioma cells to temozolomide and inhibits glioma cell invasion by targeting E2F3. PMID: 25515700
27. Data suggest that aberrant cell cycle activation in Ewing sarcoma is due to the de-repression of transcription factor E2F targets of transcriptional induction and physical recruitment of E2F3 by fusion protein EWS-FLI1 replacing E2F4 on their promoters. PMID: 25712098
28. E2F3 may be a potential target gene of miR-125a-5p in gastric cancer. PMID: 25231560
29. High E2F3 expression is associated with hepatocellular carcinoma. PMID: 25073510
30. miR-141 suppresses the growth and metastasis of HCC cells by targeting E2F3. PMID: 25142234
31. findings suggest miR-217 function as a potential tumor suppressor in HCC progression and miR-217-E2F3 axis may be a novel candidate for developing rational therapeutic strategies PMID: 24671492
32. HLM006474 synergizes with paclitaxel in lung cancer cell lines to inhibit E2F PMID: 24831239
33. novel HDAC1/4/miR-200b/E2F3 signaling contributes to chemoresistance of human lung adenocarcinoma cells PMID: 24830600
34. autoregulatory feedback loop of EZH2/miR-200c/E2F3 served an important function in prostate cancer development PMID: 25017995
35. Overexpression of E2F3 may be associated with unfavorable prognosis in hepatocellular carcinoma. PMID: 24402082
36. Wilms' tumors with aggressive features, such as higher stage, unfavorable histology and higher risk level, expressed higher levels of E2F3 protein and mRNA. PMID: 24210184
37. Studies show that a large fraction of E2F3 target genes are synergistically coregulated by aberrant ETS proteins. PMID: 23940108
38. two cell cycle-related molecules, cyclin D3 and E2F3, were identified as the direct miR-503 targets. PMID: 23967867
39. Studies revealed that E2F3 was a direct target of miR-449a in lung cancer cells. miR-449a also suppressed tumor formation in vivo in nude mice. These results suggest that miR-449a plays an important role in lung cancer tumorigenesis. PMID: 24211326
40. Overexpression of E2F3 promotes proliferation of functional human beta cells without induction of apoptosis. PMID: 23907129
41. The correlation between CCND1, E2F3 and miR-449b showed that miR-449b could downregulate CCND1 and E2F3 expression. This, in turn, reduced the proliferative ability of colon cancer stem cells. PMID: 23674142
42. down-regulation of E2f3 with age helps drive the dramatic decline in Igf2 expression in postnatal organs, and E2F3 overexpression in cancer induces IGF2 overexpression PMID: 23530192
43. Studied E2F3 and its two isoforms E2F3a and E2F3b in wild-type ovarian carcinomas and ovarian carcinomas associated with germline BRCA1 and BRCA2 mutations. PMID: 22887716
44. findings authenticate the efficiency of siRNA (E2F3) to fight against breast cancer; hence, the siRNA mediated E2F3 gene silencing knockdown the E2F3 PMID: 22960857
45. Data suggest that microRNA/mRNA pairs in hsa-miR-140-3p/RAD51AP1/, hsa-miR-145/E2F3, hsa-miR-139-5p/TOP2A, and hsa-miR-133a/GCLC were correlated with ovarian tumorigenesis. PMID: 22452920
46. Trim28 regulates cell proliferation by bridging HDAC1 and E2F3 and E2F4 interactions. PMID: 23060449
47. A homology model of DNA-binding domain of transcription factor E2F3 was generated for the design of selective ligands. PMID: 23009996
48. E2F3 controls the ubiquitination of PCNA through the transcriptional regulation of Rad18. PMID: 22391204
49. Our results suggest that down-regulation of miR-200b could lead to E2F3 overexpression and in turn contribute to chemoresistance of lung adenocarcinoma cells to docetaxel. PMID: 22139708
50. miR-203 induced cellular senescence by targeting E2F3 in human melanoma Mewo and A2058 cells PMID: 22354972
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亞細胞定位：

Nucleus.
蛋白家族：

E2F/DP family
數據庫鏈接：

HGNC: 3115

OMIM: 600427

KEGG: hsa:1871

STRING: 9606.ENSP00000262904

UniGene: Hs.269408