Recombinant Human SCL-interrupting locus protein (STIL), partial

中文名稱：

人STIL重組蛋白
貨號：

CSB-YP618008HU
規格：
來源：

Yeast
其他：

中文名稱：

人STIL重組蛋白
貨號：

CSB-EP618008HU
規格：
來源：

E.coli
其他：

中文名稱：

人STIL重組蛋白
貨號：

CSB-EP618008HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人STIL重組蛋白
貨號：

CSB-BP618008HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人STIL重組蛋白
貨號：

CSB-MP618008HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

STIL
Uniprot No.：

Q15468
別名：

DKFZp686O09161; MCPH7; OTTHUMP00000009566; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly.
基因功能參考文獻：
1. Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly. PMID: 29712910
2. Results from a study on gene expression variability markers in early-stage human embryos shows that STIL is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
3. we observed that depletion of STIL enhanced canonical homologous recombination repair and increased BRCA1 and RAD51 foci in response to DNA double strand breaks. Thus, inhibition of STIL significantly enhances the efficacy of DNA damaging chemotherapeutic drugs in treatment of ovarian cancer. PMID: 28423708
4. These data show that complementary mechanisms, such as mother-daughter centriole proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis occurs once and only once per cell cycle, raising parallels to the cell-cycle regulation of DNA replication and centromere formation. PMID: 27112295
5. RTTN directly interacts with STIL and acts downstream of STIL-mediated centriole assembly, contributing to building full-length centrioles. PMID: 28811500
6. Deletions of Stil is associated with acute T-lymphoblastic leukemia. PMID: 27759908
7. Studies indicate that depletion of any one of the protein kinase polo-like kinase 4 (PLK4) and the two proteins STIL and SAS-6 blocks centriole duplication, and, conversely, overexpression causes centriole amplification. PMID: 27911707
8. Reconstituting mouse embryonic fibroblasts lacking endogenous Stil, the authors show that STIL oligomerization mediated by these residues is not only important for the centrosomal functions of STIL during the canonical duplication process but also for de-novo formation of centrosomes. PMID: 27075531
9. Egyptian T-cell acute lymphoblastic leukemia cases seemed to have a different genetic pattern compared to other populations, with a lower incidence of TLX3/HOX11L2 and SIL/TAL but a higher incidence of NKX2-5 expression than recorded in Western countries PMID: 24571118
10. data provide evidence for novel functions of the human oncogene Stil in neural toxic susceptibility. PMID: 26549353
11. The authors suggest that the STIL-coiled-coil region/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication. PMID: 26188084
12. The STIL p.Gly717Glu mutation was not able to fully restore the centriole duplication failure following depletion of endogenous STIL protein indicating the deleterious role of the mutation. PMID: 25658757
13. Negative feedback by centriolar STIL regulates bimodal centriolar distribution of Plk4 and seemingly restricts occurrence of procentriole formation to one site on each parental centriole. PMID: 25342035
14. studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis PMID: 24052813
15. STIL mutation causes autosomal recessive microcephalic lobar holoprosencephaly. PMID: 25218063
16. propose that centriole amplification triggered by STIL stabilization is the underlying cause of microcephaly in human patients with corresponding STIL mutations PMID: 24485834
17. results provide direct evidence for the involvement of Stil in dopaminergic cell proliferation PMID: 24853807
18. A novel function for Stil protein is neural progenitor cell proliferation but not neural differentiation. PMID: 24240054
19. Identification of a novel STIL mutation in a family with primary microcephaly. PMID: 22989186
20. STIL cooperates with SAS-6 and PLK4 in the control of centriole number and represents a key centriole duplication factor in human cells. PMID: 22349698
21. These findings demonstrate that STIL is an essential component of the centriole replication machinery in mammalian cells. PMID: 22349705
22. STIL and CPAP are essential for centriole formation and for proper spindle position. PMID: 22100914
23. STIL depletion inhibited normal centriole duplication, Plk4-induced centriole amplification, and CPAP-induced centriole elongation, and resulted in a failure to localize hSAS6 and CPAP to the base of the nascent procentriole. PMID: 22020124
24. dHPLC techniques were used to screen for mutations and these studies identified several common polymorphisms but no disease-associated mutations, suggesting that SIL is not a common factor in holoprosencephaly pathogenesis in humans. PMID: 12438740
25. isolation and characterization of a cloned promoter as well as its role in leukemogenesis PMID: 12531481
26. Children with t-cell malignancies and with SIL-TAL fusion had low levels of residual disease at the end of induction and a favorable prognosis PMID: 14504110
27. Sil has a role in increased mitotic activity in tumor cells PMID: 15107824
28. Cell cycle-dependent phosphorylation of Sil is required for its interaction with Pin1, a regulator of mitosis. PMID: 16024801
29. SIL is important for the transition from the G(2) to the M phases of the cell cycle. Inducible knockdown of SIL in cancer cells in vitro delayed entrance into mitosis, decreased activation of the CDK1 (CDC2)-cyclin B complex, and induced apoptosis. PMID: 17456584
30. Mice carrying both SILloxloxSCL and Cre transgenes have increased CD4-negative/CD8-negative thymocytes compared with transgene-negative mice. PMID: 17460775
31. These data, taken together, identify SIL as a novel, vertebrate-specific regulator of mitotic spindle assembly. PMID: 17576815
32. LMO2, TAL1, Ttg-1, and SIL support levels of V(D)J recombination above background levels in cell culture and are also cleaved by the RAG proteins, while Hox11 and SCL are nicked but not cleaved efficiently in vitro PMID: 18187418
33. A role ofor SIL in derepressing GLI1 from the negative control of SUFU. PMID: 18829525
34. Primary microcephaly is an autosomal-recessive congenital disorder characterized by smaller brain size and mental retardation, in which homozygote mutations in STIL proteins are found. PMID: 19215732
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相關疾?。?/div>
Microcephaly 7, primary, autosomal recessive (MCPH7)
亞細胞定位：

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
組織特異性：

Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
數據庫鏈接：

HGNC: 10879

OMIM: 181590

KEGG: hsa:6491

STRING: 9606.ENSP00000360944

UniGene: Hs.525198