Recombinant Human Ribonuclease H1 (RNASEH1)

中文名稱：

人RNASEH1重組蛋白
貨號：

CSB-YP019801HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人RNASEH1重組蛋白
貨號：

CSB-EP019801HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RNASEH1重組蛋白
貨號：

CSB-BP019801HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人RNASEH1重組蛋白
貨號：

CSB-MP019801HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RNASEH1
Uniprot No.：

O60930
別名：

RNASEH1; RNH1; Ribonuclease H1; RNase H1; EC 3.1.26.4; Ribonuclease H type II
種屬：

Homo sapiens (Human)
蛋白長度：

Full length protein
表達區域：

1-286
氨基酸序列

MSWLLFLAHR VALAALPCRR GSRGFGMFYA VRRGRKTGVF LTWNECRAQV DRFPAARFKK FATEDEAWAF VRKSASPEVS EGHENQHGQE SEAKASKRLR EPLDGDGHES AEPYAKHMKP SVEPAPPVSR DTFSYMGDFV VVYTDGCCSS NGRRRPRAGI GVYWGPGHPL NVGIRLPGRQ TNQRAEIHAA CKAIEQAKTQ NINKLVLYTD SMFTINGITN WVQGWKKNGW KTSAGKEVIN KEDFVALERL TQGMDIQWMH VPGHSGFIGN EEADRLAREG AKQSED
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.
基因功能參考文獻：
1. RNASEH1 gene variants associate with susceptibility/protection to T1 Diabetes in Colombia. PMID: 29204916
2. Studies indicate that ribonuclease H1 is essential for mitochondrial DNA replication. PMID: 27402764
3. Data show that the catalytic domains of E. coli and human RNase H have nearly identical sequence preferences, which correlate with the efficiency of RNase H-recruiting antisense oligonucleotides. PMID: 29126318
4. Data suggest that ribonuclease H1 (RNASEH1) plays important role in replication fork movement by resolving R-loops (RNA-DNA hybrids); RNASEH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage; RNASEH1 appears to contribute to genome stability and preserves telomere integrity. PMID: 28717002
5. RPA is a sensor of R loops and a regulator of RNaseH1, extending the versatile role of RPA in suppression of genomic instability. PMID: 28257700
6. RNaseH1 maintains regulated levels of telomeric RNA-DNA hybrids at ALT telomeres to trigger homologous recombination without compromising telomere integrity too severely PMID: 25330849
7. found that the 3' fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5' ends by nuclear XRN2 after RNase H1-mediated cleavage PMID: 26159921
8. Altered RNaseH1 has a reduced capability to remove the RNA from RNA-DNA hybrids leading to impaired mtDNA replication and adult-onset mitochondrial encephalomyopathy. PMID: 26094573
9. RNase H1 and protein P32 are involved in mitochondrial pre-rRNA processing PMID: 23990920
10. data implicate the H264 side chain in phosphodiester hydrolysis as well as in product release, and are consistent with a proposed model in which the RNAse H1 H264 side chain interacts with a divalent metal ion to support catalysis PMID: 23078533
11. On the basis of its nuclear magnetic resonance (NMR) nucleic acid structure, a boranophosphonate-modified, fully R(P) BH(3) DNA/RNA hybrid is predicted not to be a substrate for RNase H1. PMID: 21443203
12. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624
13. The cysteine residues responsible for the redox-dependent activity of RNase H1 were determined by site-directed mutagenesis to involve Cys(147) and Cys(148), producing an inactive enzyme conformation by disulfide bond formation. PMID: 12473655
14. Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate PMID: 14506260
15. in human cells RNase H1 is responsible for most of the activity of DNA-like antisense drugs PMID: 14960586
16. analysis of catalytic site of human RNase H1 for heteroduplex substrate catalysis PMID: 15205459
17. method for enhancing the human RNase H1 activity of chimeric antisense oligonucleotides PMID: 17028157
18. THE role substrate structure plays in directing human RNase H1 activity as well as the design of effective antisense oligodeoxyribonucleotides. PMID: 17028158
19. Report crystal structures of RNase H1 in complex with RNA/DNA hybrids. PMID: 17964265
20. Characterization of full-length enzymes with defective hybrid binding domain indicates that this domain dramatically enhances both the specific activity and processivity of RNase H1. PMID: 18337749
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相關疾病：

Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2)
亞細胞定位：

Cytoplasm.
蛋白家族：

RNase H family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 18466

OMIM: 604123

KEGG: hsa:246243

STRING: 9606.ENSP00000313350

UniGene: Hs.568006