Recombinant Human RNA-binding protein 25 (RBM25), partial

中文名稱：

人RBM25重組蛋白
貨號：

CSB-YP019416HU
規格：
來源：

Yeast
其他：

中文名稱：

人RBM25重組蛋白
貨號：

CSB-EP019416HU
規格：
來源：

E.coli
其他：

中文名稱：

人RBM25重組蛋白
貨號：

CSB-EP019416HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人RBM25重組蛋白
貨號：

CSB-BP019416HU
規格：
來源：

Baculovirus
其他：

中文名稱：

人RBM25重組蛋白
貨號：

CSB-MP019416HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RBM25
Uniprot No.：

P49756
別名：

Arg/Glu/Asp rich protein; 120 kDa; Arg/Glu/Asp-rich protein of 120 kDa; fSAP94; functional spliceosome-associated protein 94; NET52; Protein S164; Rbm25; RBM25_HUMAN; RED120; RNA binding motif protein 25; RNA binding region (RNP1; RRM) containing 7; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding region-containing protein 7; RNPC7 ; S164 ; Snu71; U1 small nuclear ribonucleoprotein 1SNRP homolog
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
基因功能參考文獻：
1. Data suggest that RBM25 is required for the viability of multiple human cell lines, suggesting that it could play a key role in pre-mRNA splicing; a region of RBM25 spanning Lys77 binds with high affinity to SRSF2, a crucial protein in exon definition, but only when Lys77 is unmethylated. (RBM25 = RNA binding motif protein 25; SRSF2 = serine- and arginine-rich splicing factor 2) PMID: 28655759
2. Structure-guided mutagenesis reveals a positively charged nucleic-acid-binding surface in the RBM25 PWI domain PMID: 23190262
3. RED120 may function to couple splicing with mRNA 3'-end formation. PMID: 17560998
4. These data suggest a novel mode for Bcl-x(S) 5' ss activation in which binding of RBM25 with exonic element CGGGCA may stabilize the pre-mRNA-U1 snRNP through interactions with hLuc7A. PMID: 18663000
亞細胞定位：

Nucleus speckle. Cytoplasm. Note=Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
數據庫鏈接：

HGNC: 23244

OMIM: 612427

KEGG: hsa:58517

STRING: 9606.ENSP00000261973

UniGene: Hs.531106