Recombinant Cullin-2 (cul-2), partial

中文名稱：

Recombinant Cullin-2(cul-2) ,partial
貨號：

CSB-YP613752CXY
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Cullin-2(cul-2) ,partial
貨號：

CSB-EP613752CXY
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Cullin-2(cul-2) ,partial
貨號：

CSB-EP613752CXY-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Cullin-2(cul-2) ,partial
貨號：

CSB-BP613752CXY
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Cullin-2(cul-2) ,partial
貨號：

CSB-MP613752CXY
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

cul-2
Uniprot No.：

Q17390
別名：

cul-2; ccd-3; div-3; ZK520.4Cullin-2; CUL-2
種屬：

Caenorhabditis elegans
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Core component of multiple cullin-RING-based CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the CBC complex depends on the variable substrate recognition component. May function in ubiquitin-mediated degradation of CKIs to target cki-1 for degradation. CBC(zif-1) may ensure germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. As part of the CBC(fem-1) complex directs ubiquitination of tra-1. As part of the CBC(lrr-1) complex, required for the ubiquitination and dissasembly of the CMG helicase complex from chromatin at the end of DNA replication. Positive cell-cycle regulator that is required at two distinct points in the cell cycle; the G1-to-S-phase transition and mitosis. Also required for proper cytoskeletal movement and mitotic chromosome condensation.
基因功能參考文獻：
1. CUL-2 RING E3 ubiquitin-ligase acts at multiple levels to control germline development; CUL-2 may similarly regulate germline development in other organisms as well. PMID: 23555289
2. segregation of the germ plasm involves both stabilization of germline proteins in the germ line and cullin-dependent degradation in the soma PMID: 12894212
3. Fem-1 protein is a substrate-specific adaptor for a CUL-2-based ubiquitin ligase. PMID: 18502413
4. MEL-26 levels are kept low by the action of another type of ubiquitin ligase, which contains CUL-2 PMID: 19361490
亞細胞定位：

Cytoplasm. Nucleus. Note=Nuclear in oocytes.
蛋白家族：

Cullin family
組織特異性：

In adults, highly expressed in meiotic cells and oocytes. In larvae, expressed in many proliferating cell types: P cells during the L1 stage; seam cells when they divide at every molt; vulval and somatic gonad cells in late L3 and L4 stages; and intestina
數據庫鏈接：

KEGG: cel:CELE_ZK520.4

STRING: 6239.ZK520.4a

UniGene: Cel.21341