VDAC1 Recombinant Monoclonal Antibody

說明書

中文名稱：

VDAC1重組抗體
貨號：

CSB-RA025821A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, LO2 whole cell lysate, Raji whole cell lysate, Rat heart tissue, Mouse brain tissue
All lanes: VDAC1 antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 KDa
Observed band size: 31 KDa

IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA025821A0HU diluted at 1:73.125 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA025821A0HU VDAC1重組單克隆抗體是一款針對線粒體外膜通道蛋白VDAC1研發的高特異性科研工具。VDAC1作為線粒體電壓依賴性陰離子通道家族核心成員，在細胞能量代謝、鈣離子穩態調控及凋亡信號傳導中發揮關鍵作用。本抗體通過重組表達技術制備，經ELISA、Western Blot（WB）和免疫組化（IHC）多平臺驗證：在WB實驗中可清晰識別目標蛋白，推薦稀釋范圍為1:500-1:5000；在IHC應用中能準確顯示組織樣本中線粒體膜定位信號，推薦使用濃度1:50-1:200，且不同批次間檢測結果穩定一致。該產品適用于探究線粒體功能異常相關研究領域，包括能量代謝調控機制、神經退行性疾病模型中的線粒體動力學變化，以及腫瘤細胞凋亡通路研究等，為體外實驗提供高靈敏度和可靠性的檢測支持。
Uniprot No.：

P21796
基因名：

VDAC1
別名：

N2441 antibody; OMP2 antibody; POR1 antibody; hVDAC1 antibody; MGC111064 antibody; Mitochondrial Porin antibody; Outer mitochondrial membrane protein porin 1 antibody; Plasmalemmal porin antibody; Porin 31HL antibody; Porin 31HM antibody; VDAC antibody; VDAC-1 antibody; Vdac1 antibody; VDAC1_HUMAN antibody; Voltage dependent anion channel 1 antibody; Voltage dependent anion selective channel protein 1 antibody; Voltage-dependent anion-selective channel protein 1 antibody; YNL055C antibody; YNL2441C antibody
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide derived from human VDAC1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3A1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol. In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. May mediate ATP export from cells.
基因功能參考文獻：
1. VDAC1 allows Ca(2+) access to the MCU, facilitating transport of Ca(2+) to the matrix, and also from the IMS to the cytosol. Intra-mitochondrial Ca(2+) controls energy production and metabolism by modulating critical enzymes in the tricarboxylic acid (TCA) cycle and fatty acid oxidation. PMID: 29594867
2. associate dysfunction in Fe-S cluster biogenesis with cleavage of VDAC1 PMID: 29596470
3. HK1 competes with SOD1 G93A mutant from familial amyotrophic lateral sclerosis cases for binding VDAC1.SOD1 G93A mutant from familial amyotrophic lateral sclerosis cases binds VDAC1 with high affinity. PMID: 27721436
4. Study shows that silencing voltage-dependent anion channel 1 (VDAC1) expression using short interfering RNA-VDAC1 in 9 glioblastoma-related cell lines, including patient-derived cells, led to marked decreases in VDAC1 levels and cell growth. PMID: 28339833
5. VDAC1 plays an important role in dengue virus infection. PMID: 27779201
6. VDAC1 is a direct target of miR-320a in non-small cell lung cancer (NSCLC) cells, and miR-320a inhibits VDAC1 expression in NSCLC cells PMID: 27304056
7. The results of this study shown VDAC1, the Potential Target of miR-320a, is Upregulated in Response to HIV-1 Tat. PMID: 27761954
8. the present study indicated that VDAC1 may interact with HPV16 E7 to promote the malignant progression of HPV-related cervical cancer PMID: 27419626
9. Porin expression was lower in patients with heart failure with preserved ejection fraction compared to controls. PMID: 27179829
10. Studied voltage-dependent anion channel 1 (VDAC1) structure and oligomerization using an Escherichia coli cell-free protein synthesis system and bicelle crystallization. PMID: 28608415
11. In this study, molecular dynamics simulations and single-channel experiments of VDAC-1 show agreement for the current-voltage relationships of an "open" channel and they also show several subconducting transient states that are more cation selective in the simulations. We observed voltage-dependent asymmetric distortions of the VDAC-1 barrel and the displacement of particular charged amino acids. PMID: 27653481
12. VDAC1 was accumulated in the desmin highly stained area of muscle fibers of desminopathy patients. PMID: 27941998
13. work raises the interesting possibility that cholesterol-mediated regulation of VDAC1 may be facilitated through a specific binding site at the functionally important Glu(73) residue. PMID: 28396346
14. this study describes novel drug candidates with a defined mechanism of action that involves inhibition of VDAC1 oligomerization, apoptosis, and mitochondrial dysfunction. The compounds VBIT-3 and VBIT-4 offer a therapeutic strategy for treating different diseases associated with enhanced apoptosis and point to VDAC1 as a promising target for therapeutic intervention. PMID: 27738100
15. Results from the simulations show that HK2 binding restricts the movement of the VDAC1 N-terminal helix. As a result, VDAC1 is kept in the open state most of the time and probably allows a constant supply of ATP to HK2 for glycolysis. PMID: 27544294
16. The findings of this study suggest that inhibition of intracellular Ca(2+/-) overload could protect cells from damage and that VDAC1 plays a considerable role in Cr(VI)-induced liver injury. PMID: 27898307
17. Our study suggested that miR-7 suppressed the expression of VDAC1 in hepatocellular carcinoma PMID: 26831666
18. Results shows that beta-barrel of human VDAC1 embedded into a membrane is highly flexible and that Ca2+, a key regulator of metabolism and apoptosis, strongly decreases its plasticity suggesting that physiological VDAC function depends on the molecular plasticity of its channel. PMID: 27021164
19. High VDAC1 expression is associated with cervical cancer. PMID: 26716410
20. Studies using B16F10 and A375 cells genetically modified for ATF2 indicated that mitochondrial ATF2 was able to dissociate Bim from the Mcl-1/Bim complex to trigger VDAC1 oligomerization. PMID: 26462148
21. findings also suggest that VDAC1 may be a novel biomarker for gastric cancer PMID: 26646027
22. serum starvation induces CREB1 expression, in turn activating miR-320a expression, which then down-regulates VDAC1 expression to facilitate mitophagy PMID: 26472185
23. Reducing VDAC1 expression induces a non-apoptotic role for pro-apoptotic proteins in glioblastoma multiforme cancer cell differentiation. PMID: 27080741
24. The works available on VDAC cysteines support the notion that VDAC1, VDAC2, and VDAC3 proteins are paralogs with a similar pore-function and slightly different, but important, ancillary biological functions. (Review) PMID: 26947058
25. The protective effect of miR-7 is partly exerted through promoting mitochondrial function by targeting VDAC1 expression. PMID: 26801612
26. Data show that voltage-dependent anion channel 1 (VDAC1) knockout cells are resistant to AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR) modulation by itraconazole, indicating VDAC1 is the mediator of the activity. PMID: 26655341
27. Amyloid beta -mediated toxicity involves mitochondrial and plasma membrane VDAC1, leading to mitochondrial dysfunction and apoptosis induction. PMID: 26542804
28. PGC-1alpha deficiency exacerbates high glucose-induced apoptosis in human umbilical vein endothelial cells through activation of VADC1. PMID: 26191154
29. The serum lever of Alzheimer's disease were increase and the expression of VDAC1 strongly correlated with the Mini-Mental State Examination scores of the AD patients. PMID: 25502766
30. VDAC1 is involved in the process of mitochondria-mediated apoptosis by mediating the release of apoptotic proteins and interacting with anti-apoptotic proteins. (Review) PMID: 25448878
31. The functional interactions between VDAC and alpha-syn, revealed by the present study, point toward the long sought after physiological and pathophysiological roles for monomeric alpha-syn in PD and in other alpha-synucleinopathies PMID: 26055708
32. Results show that BNIP3 interacts with the voltage-dependent anion channel (VDAC) to directly induce mitochondrial release and nuclear translocation of EndoG. PMID: 25436615
33. TP53 regulation of VDAC1 cleavage occurs through mitochondrial Mieap and is dependent on the endolysosomal pH. PMID: 25691661
34. These data suggest that an interaction between Mcl-1 and VDAC promotes lung cancer cell migration by a mechanism that involves Ca(2+)-dependent reactive oxygen species production. PMID: 25341036
35. data indicate that the BH4 domain of Bcl-XL, but not that of Bcl-2, selectively targets VDAC1 and inhibits apoptosis by decreasing VDAC1-mediated Ca(2+) uptake into the mitochondria PMID: 25681439
36. Voltage-dependent structural changes of hVDAC1 PMID: 24728177
37. VDAC1 was expressed and reconstituted into two-dimensional lipid crystalline bilayers with characteristics identical to wild type samples. PMID: 25545271
38. Results indicate that mitochondrial function associated with VDAC1 is decreased in sporadic and experimental Parkinson's disease, and this decrease is associated with alpha-synuclein accumulation and aggregation PMID: 24825319
39. Data indicate that voltage-dependent anion channel 1 (VDAC1) is involved in plasminogen kringle 5 (K5)-induced activation of the mitochondrial apoptosis pathway. PMID: 25296756
40. Data indicate that curcumin interacts with residues in the alpha helical N-terminus of voltage dependent anion channel VDAC-1 and in the channel wall. PMID: 25459681
41. Ca(2+)-mediated regulation of VDAC1 expression levels is associated with cell death induction. PMID: 24704533
42. Label-free quantitative comparison of DN urinary exosomes vs control group and SRM further validation, resulted in the discovery of a panel of three proteins (AMBP, MLL3 and VDAC1) which changes in DN. PMID: 24211404
43. the C-terminus end of VDAC faces the mitochondrial inter-membrane space. PMID: 24324700
44. This review examines the significance of this new form of VDAC1 for anticancer therapy.[review] PMID: 24272356
45. Nucleotide interactions of the human voltage-dependent anion channel. PMID: 24668813
46. Increase in mRNA levels of the voltage-dependent anion channel 1 gene is associated with Alzheimer's disease. PMID: 24063855
47. Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer's disease. PMID: 22926141
48. VDAC binds tissue-type plasminogen activator (t-PA) on human neuroblastoma SK-N-SH cells PMID: 23161549
49. VDAC 1, 2, and 3 recruit Parkin to defective mitochondria to promote mitochondrial autophagy. PMID: 23060438
50. The N-terminal helix of VDAC1 controls entry into elliptic beta-barrel states which underlie VDAC closure. PMID: 22841291
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亞細胞定位：

Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Membrane raft; Multi-pass membrane protein.
蛋白家族：

Eukaryotic mitochondrial porin family
組織特異性：

Expressed in erythrocytes (at protein level). Expressed in heart, liver and skeletal muscle.
數據庫鏈接：

HGNC: 12669

OMIM: 604492

KEGG: hsa:7416

STRING: 9606.ENSP00000265333

UniGene: Hs.519320

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