ADORA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ADORA1重組抗體
貨號：

CSB-RA224168A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate
All lanes: ADORA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 14 kDa
Observed band size: 37 kDa

IHC image of CSB-RA224168A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA224168A0HU ADORA1重組單克隆抗體是針對腺苷A1受體（ADORA1）研發(fā)的高特異性檢測工具。該G蛋白偶聯(lián)受體廣泛分布于中樞神經(jīng)系統(tǒng)及外周器官，通過介導腺苷信號參與神經(jīng)調(diào)控、心血管穩(wěn)態(tài)維持和炎癥反應等生理病理過程。本抗體經(jīng)多平臺驗證顯示卓越性能：在Western blot實驗中能特異性識別天然及重組ADORA1蛋白，呈現(xiàn)單一清晰條帶；免疫組化檢測可實現(xiàn)組織樣本的精準定位，有效呈現(xiàn)受體在特定細胞類型中的分布特征；同時具備ELISA檢測所需的高親和力。優(yōu)化的稀釋方案（WB 1:500-1:5000；IHC 1:50-1:200）為研究者提供靈活的實驗選擇，嚴格的質(zhì)量控制確保批次間穩(wěn)定性。適用于探究腺苷信號通路在神經(jīng)退行性疾病、心肌保護機制、代謝調(diào)控及免疫微環(huán)境調(diào)節(jié)等領(lǐng)域的作用機制，可支持基礎(chǔ)研究中的蛋白表達分析、組織定位研究及分子互作實驗，為相關(guān)信號轉(zhuǎn)導研究提供可靠工具。
Uniprot No.：

P30542
基因名：

ADORA1
別名：

ADORA1; Adenosine receptor A1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ADORA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10D12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
基因功能參考文獻：
1. ADORA1 is not a common risk factor or causal gene for Parkinson's disease or dementia with Lewy bodies in the European population PMID: 27987235
2. monocyte-derived macrophages from ankylosing spondylitis patients expressed increased levels of A2AAR and reduced levels of A1 and A2BAR compared to healthy controls PMID: 29524036
3. studies revealed individual trait characteristics for being either vulnerable or resilient to both alcohol and to sleep deprivation. Both interventions induce gradual increases in cerebral A1AR availability, pointing to a potential common molecular response mechanism. PMID: 30012607
4. Data suggested that adenosine A1 receptor might potentiate glycinergic transmission through Galphai/PKA/alpha3 and Gbetagamma/alpha1ins pathways in inflamed rat. PMID: 28882563
5. Elevated A1 adenosine receptor is associated with sleep deprivation. PMID: 28373571
6. Mutation in ADORA1 may be associated with early-onset parkinsonism and cognitive dysfunction. PMID: 27134041
7. Inhibition of cholinergic neurotransmission by beta3-adrenoceptors results from adenosine release via equilibrative nucleoside transporters and prejunctional A1-receptor stimulation in urinary bladder. PMID: 28446460
8. This study showed that increased neuronal A1R expression in Rasmussen Encephalitis may be involved in prevention of seizures spread and seizures-induced damage, limitation of both seizures and inflammation atrophy in 1 cerebral hemisphere. PMID: 28789481
9. A1R signaling enhances A2AR-mediated neurodegeneration [review] PMID: 28441750
10. Mutational and computational analysis of A1-AR revealed a distinct conformation of the second extracellular loop and a wider extracellular cavity with a secondary binding pocket that can accommodate orthosteric and allosteric ligands. PMID: 28235198
11. second extracellular loop is a key allosteric ligand-binding determinant. PMID: 27683013
12. this study highlights a key role for extracellular loop 2 in A1AR orthosteric ligand binding and receptor activation. PMID: 27683014
13. Dysregulation in ADORA1/ADORA2A expression was associated with glioma development. PMID: 27038930
14. A1R mRNA levels and A1R density in PBMCs from idiopathic normal-pressure hydrocephalus patients were significantly lower than control subjects PMID: 26810542
15. Its central activation could be and approach to achieving a shifted homeostasis in which physiology is downregulated to a homeostatically-regulated, stable hypometabolic state. (review) PMID: 27333659
16. In postmortem human prefrontal cortex, adenosine A1 receptor is coupled preferentially, if not exclusively, to Galphai-3. PMID: 26213104
17. Results show that ADORA1 rs2228079 and ADORA2A rs5751876 polymorphisms are associated with the risk of Gilles de la Tourette Syndrome, co-morbid disorders, and may affect the age of tics onset in Polish population. PMID: 26317759
18. It has the intrinsic ability to form a heteromeric complex with metabotropic glutamate receptor type 1 and mutually modulate signaling. PMID: 26154847
19. Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A1R-mediated transactivation of EGFR. PMID: 25661317
20. Adenosine A1 receptor activation increases ENT1 activity via protein kinase C. PMID: 25725289
21. This study revealed variation of regional A1AR availability associated with ADORA1 and particularly ADORA2A SNPs, several of which had previously been shown to be associated with increased anxiety and panic disorder. PMID: 24943643
22. Stimulation of A1AR and A2BAR had a prominent anti-proliferative/pro-apoptotic effect on the glioblastoma stem cells. PMID: 25429616
23. Results document that endogenously formed adenosine can chaperone its cognate A1 receptor. PMID: 25354767
24. Results suggest that the anti-cancer effect of Omega-3 polyunsaturated fatty acids (Omega-3 PUFAs) on gastric cancer is at least partly dependent on activating the ADORA1-mediated apoptosis pathway. PMID: 24896321
25. regulation of GABA uptake occurs via modulation of the GABA transporters by the adenosine A1 (A1R) and A2A (A2AR) receptors PMID: 23657626
26. Induction of renal proximal tubular IL-11 is a critical intermediary in A1 adenosine receptor-mediated renal protection against acute ischemic kidney injury. PMID: 23813214
27. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
28. Imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of cystatin A via the adenosine receptor A1 pathway PMID: 22787201
29. A1/A2 adenosine receptor activation does not directly contribute to cutaneous active vasodilatation. PMID: 22356216
30. A(1) and A(2B)ARs could be potential biomarkers able to provide an early indication of SA risk and their stimulation may turn out to improve fetoplacental perfusion by increasing vascular endothelial growth factor (VEGF) and nitric oxide (NO). PMID: 22867902
31. In patients with stable angina ADORA1 agonist capadenoson lowers exercise heart at comparable maximum workload, which is associated with improved total exercise time and prolongation of time to ischaemia. PMID: 22370739
32. Results indicate a role for adenosine receptor A1 and A2A gene polymorphisms in susceptibility to apnoea of prematurity and bronchopulmonary dysplasia and in interindividual variability to caffeine response. PMID: 22462821
33. Data suggest that expression of ADORA1 is too low to play a major role in regulation of lipogenic gene expression in hepatocytes; studies include investigation of gene expression under influence of ADORA1 agonists. PMID: 22449383
34. data show that expression of A1Rs induced adipocyte differentiation, whereas A2bR expression inhibited adipogenesis and stimulated an osteoblastic phenotype PMID: 21730968
35. AMP and a non-hydrolyzable AMP analog (deoxyadenosine 5'-monophosphonate, ACP) directly activated the adenosine A(1) receptor PMID: 22215671
36. Inhibition of human mast cell activation would be a mechanism for A AR antagonists, but not A(2B) AR antagonists. PMID: 21506953
37. Adenosine receptor expression and activation during the differentiation of mesenchymal stem cell to osteoblasts and adipocytes, was investigated. PMID: 21590734
38. two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in epicardial adipose tissue compared to mediastinal and subcutaneous adipose tissue PMID: 21603615
39. Data suggest that adenosine deficiency may be relevant to human epilepsy and that a ketogenic diet can reduce seizures by increasing adenosine A1 receptor-mediated inhibition. PMID: 21701065
40. ADORA1 was expressed in nucleus, perinucleus and cytoplasm of retinal pigment epithelium. PMID: 21542986
41. report implicating genetic variability in the A1AR with post-traumatic seizures. PMID: 20609566
42. LUF5834 represents a novel high affinity radioligand for the A(1)R and may prove a useful tool to provide estimates of inverse agonist efficacy at this receptor. PMID: 20599769
43. results suggest that adenosine-mediated signaling in the heart requires a balance between A(1)- and A(2A)-receptors PMID: 20354569
44. Report fluorescence-based techniques, and in particular the development of fluorescent ligands, so study pharmacology of the adenosine A1 receptor. PMID: 20105183
45. Findings suggest that the adenosine A1 (and possibly coordinated with A2a) receptors contribute to dendritic cell differentiation and survival. PMID: 20085598
46. Studies indicate that adenosine mediates its actions by means of activation of specifiic G protein-coupled receptors, for which 4 subbtypes: A1R, A2AR, A2BR and A3R have been identified so far. PMID: 19883624
47. Adenosine, thus, suppresses CW2 human colonic cancer growth by inducing apoptosis as mediated via A(1) adenosine receptors. PMID: 19822392
48. Data suggest that adenosine receptor modulation may be useful for refining the use of chemotherapeutic drugs to treat human cancer more effectively. PMID: 19794965
49. ADORA1 polymorphisms may represent good candidate markers for schizophrenia research and ADORA1 may be involved in the pathophysiological mechanisms of schizophrenia in Japanese populations PMID: 19820430
50. Human A1 adenosine receptor overexpressed in adipose tissue of transgenic mice protects them from obesity-related insulin resistance. PMID: 11703426
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
數(shù)據(jù)庫鏈接：

HGNC: 262

OMIM: 102775

KEGG: hsa:134

STRING: 9606.ENSP00000308549

UniGene: Hs.77867

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