USP22 Recombinant Monoclonal Antibody

說明書

中文名稱：

USP22重組抗體
貨號：

CSB-RA784212A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate
All lanes: USP22 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 59 kDa
Observed band size: 55-72 kDa

Immunofluorescence staining of Hela cell with CSB-RA784212A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 507-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA784212A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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產品詳情
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靶點詳情

產品詳情

產品描述：

USP22重組單克隆抗體（CSB-RA784212A0HU）靶向去泛素化酶USP22，該蛋白通過調控組蛋白H2B的去泛素化修飾參與表觀遺傳調控、細胞周期進程及腫瘤發生等關鍵生物學過程。本抗體經多種實驗驗證，適用于ELISA、Western Blot、免疫熒光（IF）和流式細胞術（FC），推薦使用稀釋度分別為WB 1:500-1:2000、IF 1:50-1:200、FC 1:50-1:200。Western Blot實驗顯示抗體能特異性識別內源性USP22蛋白，免疫熒光結果清晰定位細胞核內信號，流式檢測驗證其適用于細胞表面及胞內標記分析。該產品為科研領域提供高靈敏度和特異性的檢測工具，可應用于腫瘤生物學研究、細胞周期調控機制探索以及蛋白質翻譯后修飾相關實驗，尤其在探究USP22在基因表達調控、干細胞分化及癌癥代謝重編程中的作用時具有重要價值。抗體采用重組技術生產，具備優異的批次間一致性，適用于多種樣本類型檢測需求。
Uniprot No.：

Q9UPT9
基因名：

USP22
別名：

Ubiquitin carboxyl-terminal hydrolase 22 (EC 3.4.19.12) (Deubiquitinating enzyme 22) (Ubiquitin thioesterase 22) (Ubiquitin-specific-processing protease 22), USP22, KIAA1063 USP3L
反應種屬：

Human
免疫原：

A synthesized peptide derived from human USP22
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

18H6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
基因功能參考文獻：
1. USP22 and CCND1 levels correlate in patient lung and colorectal cancer samples and our preclinical studies indicate that targeting USP22 in combination with CDK inhibitors may offer an approach for treating cancer patients whose tumors exhibit elevated CCND1. PMID: 30224477
2. these findings suggest that miR-30e-5p suppresses non-small cell lung cancer (NSCLC) tumorigenesis by downregulating USP22-mediated Sirt1/JAK/STAT3 signaling. PMID: 29174979
3. USP22 played an important role in retinoblastoma cell proliferation/aging and apoptosis. PMID: 28682440
4. indicate USP22 as a novel deubiquitinase of BMI1 in glioma PMID: 29788550
5. USP22 mediates CRC cell chemoresistance through the Wnt/beta-catenin pathway and that reducing USP22 in CRC cells diminishes chemoresistance. PMID: 29689565
6. Data provided evidence that USP22, an up-stream molecule of AP4, exhibits strong potential to promote colorectal cancer (CRC) metastasis, particularly CRC migration and invasion capacities, both in vitro and in vivo, by inducing EMT via AP4 activating. Moreover, USP22 and AP4 overexpression may stimulate tumor metastasis and adversely affect overall survival in CRC patients. PMID: 28427243
7. Cancer stem cells marker USP22 influences drug sensitivity via regulating SIRT1, which will shed new insights into the mechanisms of multidrug resistance in hepatocellular carcinoma . PMID: 28417539
8. our study demonstrates that USP22 is indispensable for gastric cancer stem cell self-renewal through stabilization of BMI1. PMID: 28415621
9. Downregulation of USP22 in ATC cells impeded tumor growth and lung metastasis in vivo. PMID: 27145278
10. Studies indicate that aberrant expression of the Ubiquitin-Specific Peptidase 22 (USP22) has been associated with poor cancer prognosis. PMID: 27057639
11. we demonstrated that USP22 was highly expressed in OS tissues and cells lines. Downregulation of USP22 inhibited OS cell proliferation, invasion, and EMT in vitro. In addition, downregulation of USP22 suppressed OS tumor growth and metastasis in vivo. PMID: 27983930
12. In breast cancer cell lines USP22 increases c-Myc stability through c-Myc deubiquitination, which is closely correlated with breast cancer progression. PMID: 28160502
13. findings suggest that USP22 may be involved in hepatocellular carcinoma progression in cooperation with survivin. PMID: 26497847
14. These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor hepatocellular carcinoma prognosis PMID: 25909224
15. USP22 attenuated the invasion capacity of colon cancer cells by inhibiting the STAT3/MMP9 signaling pathway. PMID: 25902005
16. Our data indicated that USP22 may promote lung adenocarcinoma cell invasion by the induction of EMT. PMID: 25907317
17. Data show that the aggregates formed by polyQ-expanded ataxin 7 sequester ubiquitin-specific protease (USP22) through specific interactions. PMID: 26195632
18. the deubiquitinating enzyme activity of USP22 is necessary for regulating HeLa cell growth, and it promotes cell proliferation via the c-Myc/cyclin D2, BMI-1 and p53 pathways in HeLa cells PMID: 26143114
19. ShRNA-mediated silencing of the ubiquitin-specific protease 22 gene restrained cell progression and affected the Akt pathway in nasopharyngeal carcinoma. PMID: 25482932
20. Data indicate that ubiquitin specific peptidase 22 (USP22)-mediated sirtuin 1 (SIRT1) deubiquitination inhibits STAT3 transcription factor acetylation and its transcriptional activation. PMID: 24969755
21. USP22 is overexpressed in human NSCLC tissues and cell lines. USP22 silencing downregulates MDMX protein expression and activates the p53 pathway. PMID: 25547493
22. Increased USP22 expression in colon cancer correlated with reduced uH2B expression, and this expression pattern may contribute to tumor progression. PMID: 25971547
23. findings of the present study suggest a potential mechanism underlying the oncogenic role of USP22 mediated by the modulation of the stability and activity of COX-2 PMID: 25817787
24. USP22 may accelerate ovarian cancer cell cycle progression via synergizing with TGFB1 to regulate the TGFB1 downstream cell cycle pathway. PMID: 25369910
25. Collectively, the present study demonstrated a new function of USP22 that induces autophagy, thus leading to the poor prognosis of pancreatic cancer. PMID: 25241857
26. Results show that USP22 and FoxM1 are overexpressed in patients with pancreatic cancer and jointly involved in the development and progression of pancreatic cancerthe disease. PMID: 24993031
27. USP22 is involved in the carcinogenesis of human pharyngeal squamous cell carcinoma. PMID: 25241842
28. The overexpression of USP22 was observed to attenuate TSAinduced apoptosis in HeLa cells. PMID: 25323692
29. These results suggest that USP22 positively regulates RCAN1 levels, which would consequently affect diverse RCAN1-linked cellular processes. PMID: 25546086
30. Overexpression of USP22 in pancreatic cancer promoted cytoskeletal remodeling, upregulated expression of transcription factors to promote epithelial-mesenchymal transition, and increased cellular invasion and migration. PMID: 25070659
31. USP22 expression may play an important role in gastric carcinoma tissue. PMID: 25445209
32. Genetic studies indicate that Gcn5 and USP22 have important roles during development, which may presage important functions for these proteins in human diseases. [review] PMID: 25111486
33. USP22 overexpression may be associated with poor prognosis in patients with glioma PMID: 24573640
34. High expression of USP22 was associated with Salivary Adenoid Cystic Carcinoma. PMID: 24466336
35. In the present study, a functional NLS and the minimal sequences required for the active targeting of USP22 to the nucleus were identified. PMID: 24802393
36. Findings define USP22 as a critical effector of tumor progression, which drives lethal phenotypes. PMID: 24197134
37. USP22 deubiquitinates and stabilizes NFATc2 protein levels thereby promoting IL2 expression. PMID: 24561192
38. In this study, we investigated the protein expression of USP22 in different cervical tissues by immunohistochemical staining and analyze the correlation between USP22 level and clinicopathologic features including patient outco PMID: 23979981
39. Overexpression of USP22 may contribute to the progression of SDC and thus may serve as a new molecular marker to predict the prognosis of SDC patients PMID: 23664741
40. USP22 plays an important role in NSCLC progression at the early stage, and that overexpression of USP22 in tumor tissues could be used as a potential prognostic marker for patients with early clinical stage of NSCLC PMID: 23361242
41. High USP22 expression is associated with papillary thyroid carcinoma. PMID: 23412977
42. Sp1 is a crucial regulator of USP22 transcription. PMID: 23300749
43. study identified the deubiquitinating enzyme ubiquitin-specific protease 22 (USP22), a component of the deubiquitinating module (DUBm) of the SAGA transcriptional coactivating complex, as a SIRT1-interacting partner PMID: 23382074
44. The USP22 regulates the cell cycle via the c--Myc/cyclin D2 pathway and down--regulating p15 and p21 expression in HepG2 cell. PMID: 23217440
45. This is the first study that determines the relationship between USP22 expression and prognosis in oral squamous cell carcinoma. PMID: 22880026
46. Data show that USP22 protein plays an essential role in esophageal squamous cell carcinoma (ESCC) progression and has clinical potentials as a biomarker and as an attractively therapeutic target for ESCC. PMID: 22447106
47. USP22 may act as an oncogene in CRC as it positively regulates cell cycle via both BMI-1-mediated INK4a/ARF pathway and Akt signaling pathway. PMID: 21928107
48. USP22 plays a crucial role in tumor formation and growth by regulating cell proliferation with USP22-dependent signaling pathway. PMID: 21773699
49. RNAi-mediated knockdown of the ubiquitin hydrolase, USP22, results in 2-fold higher ubH2B, and 2-fold lower transcriptional elongation at IRF1. USP22 depletion also diminishes 3'-end cleavage/polyadenylation by 2- to 3-fold. PMID: 22067483
50. simultaneous activation of USP22 and BMI-1 may associate with GC progression and therapy failure PMID: 21735131
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亞細胞定位：

Nucleus.
蛋白家族：

Peptidase C19 family, UBP8 subfamily
組織特異性：

Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
數據庫鏈接：

HGNC: 12621

OMIM: 612116

KEGG: hsa:23326

STRING: 9606.ENSP00000261497

UniGene: Hs.462492

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