SAFB Recombinant Monoclonal Antibody
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中文名稱：SAFB Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA035113A0HU
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規(guī)格：￥1320
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圖片：
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Flow cytometric analysis of scaffold attachment factor B expression in HeLa cells using scaffold attachment factor B antibody. Green, isotype control; red, scaffold attachment factor B. -
Immunocytochemical staining of HeLa cells with Scaffold attachment factor B antibody. Nuclei were stained blue with DAPI; Scaffold attachment factor B was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. -
Western blotting analysis using scaffold attachment factor B antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with scaffold attachment factor B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：SAFB
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別名：SAFB; Scaffold Attachment Factor B; SAFB1; HET; HSP27 Estrogen Response Element-TATA Box-Binding Protein; Hsp27 ERE-TATA Binding Protein; HSP27 ERE-TATA-Binding Protein; Scaffold Attachment Factor B1; SAF-B1; SAF-B; HAP; Heat-Shock Protein (HSP27) Estrogen Response Element And TATA Box-Binding Protein; Glutathione S-Transferase Fusion Protein; SAB-B1
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反應(yīng)種屬：Human, Mouse, Rat
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免疫原：Recombinant Human SAFB protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：9C2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, FC, ICC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.
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基因功能參考文獻(xiàn)：
- SAFB regulated the activity of NF-kappaB signaling in CRC by targeting TAK1 This novel mechanism provides a comprehensive understanding of both SAFB and the NF-kappaB signaling pathway in the progression of CRC and indicates that the SAFB-TAK1-NF-kappaB axis is a potential target for early therapeutic intervention in CRC progression PMID: 28912140
- Depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. Moreover, FUS interacts with another nuclear matrix-associated protein, Matrin3. PMID: 27731383
- Data suggest that ERH interacts directly in nucleus with C-terminal Arg-Gly-rich region of SAFB1/SAFB2 and this multimer co-localizes in insoluble nuclear fraction; binding of ERH reverses inhibition exerted by SAFB1/SAFB2 on SRPK1. (ERH = enhancer of rudimentary homolog protein; SAFB = scaffold attachment factor B; SRPK1 = splicing kinase SR protein kinase-1) PMID: 28627136
- The expression of coding and non-coding genes with SAFB1 cross-link sites was altered by SAFB1 knockdown. The isoform-specific expression of neural cell adhesion molecule (NCAM1) and ASTN2 was influenced by SAFB1. PMID: 26694817
- Single depletion of either SAFB1 or SAFB2 leads to an increase in expression of the other SAFB protein. PMID: 26273616
- reveals an unexpected role of SUMO-1 and SAFB in the stimulatory coupling of promoter binding, transcription initiation and RNA processing PMID: 25800734
- SAFB1 formed a complex with the histone methyltransferase EZH2. PMID: 23893242
- Data indicate that scaffold attachment factor SAFB1 is transiently recruited to DNA breaks in a poly(ADP-ribose)-polymerase 1- and poly(ADP-ribose)-dependent manner. PMID: 24055346
- Results indicate that SAFB1 and SAFB2 are crucial repressors for ERalpha dynamics in association with the nuclear matrix and that their synergistic regulation of ERalpha mobility is sufficient for inhibiting ERalpha function. PMID: 22566185
- transcriptional repressor SAFB1 is modified by both SUMO1 and SUMO2/3, and this modification is necessary for its full repressive activity. PMID: 21527249
- Data show that binding of p53 to SAFB1 had a significant functional outcome, since SAFB1 was shown to suppress p53-mediated reporter gene expression. PMID: 21130767
- This study shows that low SAFB protein levels predict poor prognosis of breast cancer patients, suggesting critical functions of SAFB1 and SAFB2 in breast cancer cells. PMID: 19137425
- Study confirms the primary role of SAFB1/SAFB2 as corepressors and also uncovers a previously unknown role for SAFB1 in the regulation of immune genes and in estrogen-mediated repression of genes. PMID: 19901029
- HSP27 is a nuclear speckle component in unstressed cells in tissue culture. It is also associated with the nucleolar compartment. PMID: 12837281
- REVIEW: possibility that SAFB1 and SAFB2 are novel breast tumor suppressor genes, and how they might function in this role, are discussed PMID: 14587024
- HSP27 expression may have useful diagnostic use for the prognosis of mouth squamous cell carcinoma. PMID: 14702179
- SAFB1 represses ERalpha activity via indirect association with histone deacetylation and interaction with the basal transcription machinery PMID: 15066997
- SAFB1 was shown to interact directly with the nuclear receptor corepressor N-CoR. PMID: 16195251
- SAFB1 interacts in pull-down assays not only with PPARgamma but also with all nuclear receptors tested PMID: 16326836
- SAFB may direct the reorganization and segregation of nuclear RNA and DNA prior to endonuclease-mediated DNA cleavage. PMID: 17643427
- Over expression of HSP27 is associated with intrahepatic cholangiocarcinoma PMID: 18154639
- hXOR is a tumor suppressor-targeted gene and the phosphorylation of SAFB1 is regulated by OSM, which provides a molecular basis for understanding the role of SAFB1-regulated hXOR transcription in cytokine stimulation and tumorigenesis PMID: 18772145
- SAFB1 is not likely to be causative of the hereditary breast cancer syndrome in west Swedish breast cancer families PMID: 19077293
- Importance of ERE-BP as an attenuator of normal ERalpha signaling in vivo and is a novel target for modulation by selective estrogen receptor modulators. PMID: 19106221
- The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2. PMID: 19674106
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亞細(xì)胞定位：Nucleus.
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組織特異性：Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
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