HIP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

HIP1 Recombinant Monoclonal Antibody
貨號：

CSB-RA158142A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of Huntingtin interacting protein 1 expression in C2C12 cells using Huntingtin interacting protein 1 antibody. Green, isotype control; red, Huntingtin interacting protein 1.

Immunocytochemical staining of C2C12 cells with Huntingtin interacting protein 1 antibody . Nuclei were stained blue with DAPI; Huntingtin interacting protein 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using Huntingtin interacting protein 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Huntingtin interacting protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

O00291
基因名：

HIP1
別名：

HIP1; Huntingtin Interacting Protein 1; ILWEQ; Huntingtin-Interacting Protein 1; Huntingtin-Interacting Protein I; HIP-I; SHONgamma; SHONbeta; HIP-1; SHON
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human HIP1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

18C11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Regulates presynaptic nerve terminal activity. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors.
基因功能參考文獻：
1. HIP1 gene expression might serve as a reliable predictor for overall survival in acute myeloid leukemia patients. PMID: 28452374
2. After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR PMID: 29039605
3. Data suggest that GLP1R signaling in pancreatic beta-cells leading to insulin secretion involves interactions of GLP1R with HIP1, SNX1, and SNX27; HIP1 appears to regulate coupling of cell surface GLP1R activation with endocytosis; SNX1 and SNX27 appear to control balance between GLP1R plasma membrane recycling and lysosomal degradation. PMID: 29284659
4. HIP1 deletion is not involved in Williams-Beuren syndrome. PMID: 26437767
5. Huntingtin-interacting protein-1 (HIP1) is known to play a role in tumorigenesis. metastasis. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201412-2226OC#.V8DF69LrtNs PMID: 26595459
6. SHON is a novel human oncogene with predictive utility in ER(+) breast cancer, perhaps offering a simple biomarker to predict the therapeutic efficacy of antiestrogen therapy in patients with breast cancer. PMID: 24296488
7. SHON plays an important role in EMT and contributes to breast cancer progression. PMID: 25082541
8. HIP1-ALK, a novel fusion protein is associated with lung adenocarcinoma. PMID: 24518094
9. HIP1-ALK-rearranged tumor is sensitive to treatment with crizotinib, implicating HIP1-ALKas an oncogenic driver of lung tumorigenesis PMID: 24496003
10. Identified a four-tyrosine "HIP1 phosphorylation motif" in the N-terminal region of HIP1 that is required for phosphorylation mediated by both EGFR and PDGFbetaR but not by the oncoproteins HIP1/PDGFbetaR (H/P), and TEL/PDGFbetaR (T/P). PMID: 23836884
11. Three neuronal proteins (Huntingtin interacting protein 1, neurofascin, and olfactomedin-like 2a) are novel components of podocyte major processes and their expression in glomerular crescents supports their role in crescent formation. PMID: 22913984
12. flexibility of the HIP1 coiled-coil domain is important for normal function and may lead to new insights into Huntington's disease PMID: 22835334
13. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
14. Huntingtin-interacting protein 1 is a Merkel cell carcinoma marker that interacts with c-Kit PMID: 21697888
15. data characterize a neurodevelopmental and epilepsy syndrome that is likely caused by recurrent and nonrecurrent deletions, including HIP1 PMID: 21109226
16. hydrogen-bond network and changes in coiled-coil monomer interaction suggest that the HIP1 coiled-coil domain is uniquely suited to allow conformational flexibility. PMID: 20179344
17. Results show that pro-apoptotic Hippi-Hip-1 heterodimers can recruit procaspase-8 into a complex of Hippi, Hip-1 and procaspase-8, and launch apoptosis through components of the 'extrinsic' cell-death pathway. PMID: 11788820
18. HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain. PMID: 11889126
19. huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival PMID: 12163454
20. both HIP1r and HIP1 bind inositol lipids via their epsin N-terminal homology (ENTH) domains. PMID: 14732715
21. HIP1 has roles in endocytosis and receptor trafficking and in tumorigenesis PMID: 15059611
22. roles of Hip1 and Hip1R in affecting clathrin assembly and actin distribution are mediated by their interaction with the conserved sequence of clathrin light chain PMID: 15533940
23. The F-actin binding capacity of Hip1 is regulated by intrasteric occlusion of primary actin-binding determinants within the Hip1 I/LWEQ module. PMID: 15581353
24. HIP1 is a nucleocytoplasmic protein capable of associating with membranes and DNA response elements and regulating transcription. PMID: 16027218
25. neurotoxic signals of Hip1 transmit through the intrinsic mitochondrial apoptotic pathwaysand the formation of apoptosome complex PMID: 16979168
26. Human HIP1 transgenic Hip1/Hip1r knockout mice are completely free from dwarfism and spinal defects. PMID: 17452370
27. Crystal structure at 2.8 A of HIP1 coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI). PMID: 18155047
28. Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain PMID: 18790740
29. Data demonstrate HIP1's role in pits maturation and formation of the coated vesicle, and its strong dependence on clathrin for membranal localization. PMID: 19626275
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相關疾病：

A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB (PubMed:9616134). The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes (PubMed:9616134). The reciprocal PDGFRB-HIP1 transcript is not expressed (PubMed:9616134).
亞細胞定位：

Cytoplasm. Nucleus. Endomembrane system. Cytoplasmic vesicle, clathrin-coated vesicle membrane. Note=Shuttles between cytoplasm and nucleus. Nuclear translocation can be induced by AR.
蛋白家族：

SLA2 family
組織特異性：

Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.
數據庫鏈接：

HGNC: 4913

OMIM: 601767

KEGG: hsa:3092

STRING: 9606.ENSP00000336747

UniGene: Hs.329266

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