MGAT5 Antibody, Biotin conjugated

說明書

中文名稱：

MGAT5兔多克隆抗體, Biotin偶聯
貨號：

CSB-PA600992LD01HU
規格：

￥880
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MGAT5 Polyclonal antibody
Uniprot No.：

Q09328
基因名：

MGAT5
別名：

6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody; 6-N-acetylglucosaminyltransferase antibody; Alpha 1,3(6) mannosylglycoprotein antibody; Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase antibody; Alpha mannoside beta 1,6 N acetylglucosaminyltransferase antibody; Alpha-1 antibody; Alpha-mannoside beta-1 antibody; Beta 1,6 N acetyl glucosaminyltransferase antibody; GGNT5 antibody; GlcNAc T V antibody; GlcNAc-T V antibody; GNT V antibody; GNT VA antibody; GNT-V antibody; GNTV antibody; GNTVA antibody; Mannoside acetylglucosaminyltransferase 5 antibody; Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase antibody; Mgat5 antibody; MGT5A_HUMAN antibody; N acetylglucosaminyl transferase V antibody; N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein antibody; N-acetylglucosaminyl-transferase V antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A protein (107-217AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Biotin
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2. Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation. Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis.; Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.
基因功能參考文獻：
1. The best homology model is consistent with available experimental data. The three-dimensional model, the structure of the enzyme catalytic site and binding information obtained for the donor and acceptor can be useful in studies of the catalytic mechanism and design of inhibitors of GnT-V. PMID: 26821880
2. Our data suggest that oxidative stress induces the overexpression of MGAT5 via the regulation of the focal adhesion kinase-extracellular signal-regulated kinase signaling pathway, which, in turn, affects the function of endothelial cells, which then participates in the pathogenesis of preeclampsia. PMID: 27334383
3. PTPalpha is identified as a novel substrate of N-Acetylglucosaminyltransferase V (GnT-V) and could be a factor regulating promotion of migration in breast cancer cells PMID: 27965091
4. Tunicamycin, an inhibitor of N-glycan biosynthesis, was also able to enhance the radiosensitivity of U251 cells. Thus, our results suggest that development of therapeutic approaches targeting N-linked beta1,6-GlcNAc branches which are encoded by N-acetylglucosaminyltransferase V may be a promising strategy in glioblastoma treatment PMID: 26526581
5. the knockdown of GnTV significantly suppressed the proliferation, migration and invasion (P<0.05) of the SMMC7721/R cells. PMID: 26531171
6. role in the inhibition of trophoblast cell invasion and migration during early pregnancy by direct or indirect regulation of MMP2/9 activity PMID: 26349781
7. the level of TGFBR1 and early osteogenic differentiation were abolished in the DPSCs transfected with siRNA for GnT-V knockdown...GnT-V plays a critical role in the hexosamine-induced activation of TGF-b signaling and osteogenic differentiation PMID: 26583147
8. Mgat5 plays an important role in early spontaneous miscarriage in humans. PMID: 26109616
9. Gnt-V caused tumour growth more quickly PMID: 26293457
10. High expression of GnT-V was observed in infiltrating cells in skin section samples from systemic and localized patients with scleroderma. PMID: 25876794
11. MGAT5 protein and gene expression in in uveal and cutaneous melanoma cells PMID: 26098720
12. Human Mgat5 increases amino acid uptake, intracellular levels of glycolytic and TCA intermediates, as well as HEK293 cell growth. PMID: 25395405
13. Study disclose that a deficiency in branched N-glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis. PMID: 24334766
14. The results identified Mgat5-mediated beta-1-6-GlcNAc branched N-glycosylation and following activation of EGFR as a potential novel upstream molecular event for PAK1-induced anoikis resistance in hepatoma cells. PMID: 23811795
15. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications. PMID: 24399258
16. GnT-V plays a significant role in metastasis and invasion in gastric cancer cells. PMID: 23563846
17. The combination of intratumoral MGAT5 expression and TNM or Kiel staging systems had a better predictive power for overall survival. PMID: 23107376
18. GnT-V directs cancer progression by modulating MMPs in cancer. PMID: 23357422
19. The results of this study have shown that the MGAT5 intronic variants rs4953911 and rs3814022 correlate with lower N-glycan branching, reduced surface CTLA-4 in human CD4 + T cell blasts, and associate with multiple sclerosis. PMID: 23351704
20. Data show that knockdown of CD147 inhibited MMP-2 activity of GnT-V-overexpressing cells, indicating that aberrant beta1,6-branches on CD147 is crucial for the induction of MMPs (matrix metalloproteinases) in SMMC-7721 cells. PMID: 23005037
21. The results suggest that GnT-V may be a potential target for predicting nasopharyngeal carcinoma response to radiotherapy. PMID: 22780953
22. These data suggested that GnT-V expression was positively related with malignancy in human hepatocellular carcinoma (HCC) and GnT-V may be both a differentiation marker and a potential target for the treatment of HCC. PMID: 22537550
23. 33 directly measured and 13 derived glycosylation traits in 3533 individuals were identified and three novel gene association (MGAT5, B3GAT1 and SLC9A9) were identified using an additional European cohort. PMID: 21908519
24. Inhibiting expression of N-acetylglucosaminyltransferase V inhibits the proliferation of PC-3 cells. PMID: 20584650
25. GnT-V expression is positively correlated with malignancy in nasopharyngeal carcinoma cells PMID: 21676538
26. Findings suggest Mgat5 may play an important role during oncogenesis, identifying a potential therapeutic target for pulmonary adenocarcinoma. PMID: 21631992
27. The rs1257169(G) allele of MGAT5 is associated with lower disease severity in multiple sclerosis PMID: 21115203
28. The GnT-V was fully active without exogenous cation and in the presence of EDTA, and pH optimum for GnT-V was in the range of 6.5-7.0. PMID: 19846580
29. Our results suggest that GnT-V could decrease human hepatoma SMMC-7721 cell adhesion and promote cell proliferation partially through RPTPkappa. PMID: 19911372
30. Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching PMID: 11864986
31. A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity. PMID: 11872751
32. Low GnT-V expression is associated with shorter survival and poor prognosis in pStage I overall Non-small cell lung cancer. PMID: 15014031
33. Data describe the effect of N-acetylglucosaminyltransferase V on the expressions of other glycosyltransferases involved in the synthesis of surface sialyl Lewis X antigen. PMID: 15044007
34. GlcNAc-transferase-V activity is up-regulated in RA and Vit-D(3)-treated hepatoma cell lines. PMID: 15313475
35. GnT-V would contribute to placentation in the early phase of pregnancy, possibly regulating the process of invasion of trophoblast cells PMID: 15809094
36. These results supported the mechanism that blocking of GnT-V expression impaired functions of chaperones and N-glycan-synthesizing enzymes, which caused UPR in vivo. PMID: 16467879
37. GnT-V is closely related to low malignant potential and good prognosis of the patients with bladder cancer. PMID: 16638859
38. Glycosylation caused by GnT-V directs integrin beta1 stability and more delivery to plasma membrane, subsequently promotes Fn-based cell migration and invasion. PMID: 16924681
39. glycosylation change & decrease of transport activity of GLUT1 may be 1 possible mechanism of ER stress induced by down-regulating GnT-V, & GnT-V may contribute to the regulation of glucose uptake by modifying glycosylation of GLUT1 in some tumor cells. PMID: 17451637
40. We investigated mRNA levels of glycosyltransferases, namely GnT-V, and found that it's expression was decreased in HLE-cells resistant to Epirubicin as well as in HLE-cells resistant to Mitoxantrone. PMID: 17488527
41. Tissue inhibitor of metalloproteinase-1 (TIMP-1), was identified as a target protein for GnT-V in human colon cancer cell WiDr. PMID: 17878270
42. Results suggested that high GnT-V expression was correlated with an unfavourable clinical outcome. PMID: 17971775
43. Knock-down of MGAT5 in PC-3 cells attenuated the metastatic ability of prostate cancer cells, as determined by the in vitro invasion assay and the xenograft animal studies. PMID: 18649738
44. GnT-V expression is correlated with a poor prognosis in gastric cancer patients due to metastases. PMID: 18931531
45. decreased GnT-Va activity due to siRNA expression in human carcinoma cells inhibits ligand-induced EGFR internalization, consequently resulting in delayed downstream signal transduction and inhibition of the EGF-induced, invasiveness-related phenotypes. PMID: 19225046
46. overexpression of GnT-V in a hepatoma cell line not only induced the addition of beta1,6 GlcNAc branch to N-glycan of RPTPkappa but also decreased the protein level of RPTPkappa PMID: 19236842
47. High expression of N-acetylglucosaminyltransferase V is associated with mucinous tumors of the ovary. PMID: 19787216
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亞細胞定位：

Golgi apparatus membrane; Single-pass type II membrane protein.; [Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A]: Secreted.
蛋白家族：

Glycosyltransferase 18 family
數據庫鏈接：

HGNC: 7049

OMIM: 601774

KEGG: hsa:4249

STRING: 9606.ENSP00000281923

UniGene: Hs.4988

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