CBX4 Antibody

中文名稱：

CBX4兔多克隆抗體
貨號：

CSB-PA004600EA01HU
規格：

￥440
圖片：
IHC image of CSB-PA004600EA01HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA004600EA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA004600EA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CBX4 Polyclonal antibody
Uniprot No.：

O00257
基因名：

CBX4
別名：

CBX 4 antibody; CBX4 antibody; Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody; CBX4_HUMAN antibody; Chromobox homolog 4 (Pc class homolog; Drosophila) antibody; Chromobox homolog 4 antibody; Chromobox protein homolog 4 antibody; E3 SUMO protein ligase CBX 4 antibody; E3 SUMO protein ligase CBX4 antibody; E3 SUMO-protein ligase CBX4 antibody; hPc 2 antibody; hPc2 antibody; NBP 16 antibody; NBP16 antibody; NS5ATP1 binding protein 16 antibody; Pc 2 antibody; Pc class 2 homolog antibody; Pc class homolog antibody; Pc class homolog Drosophila antibody; PC2 antibody; Polycomb 2 homolog antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human E3 SUMO-protein ligase CBX4 protein (1-160AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CBX4 Antibody (CSB-PA004600EA01HU)，的標記方式是Non-conjugated。對于CBX4 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA004600EB01HU	CBX4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA004600EC01HU	CBX4 Antibody, FITC conjugated
Biotin	CSB-PA004600ED01HU	CBX4 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.; Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3). Plays a role in the lineage differentiation of the germ layers in embryonic development.
基因功能參考文獻：
1. Data suggest that CBX4 expression is up-regulated in breast cancer and is correlated with unfavorable overall survival; CBX4 promotes cell growth and migration via transcriptionally suppressing expression of miR137 to trigger Notch1 signaling pathway. (CBX4 = chromobox homolog 4; miR137 = microRNA 137; Notch1 = neurogenic locus notch homolog protein-1) PMID: 29229426
2. Data show that USP26 interacts with PRC1 components chromobox (CBX)-containing proteins CBX4 and CBX6. PMID: 28839133
3. Data show that SUMO E3 ligase CBX4 sumoylates subpopulation of CtIP to regulate recruitment to breaks and resection. PMID: 28740167
4. provide evidence to show that CBX4 may serve as a tumor suppressor in colorectal carcinoma by recruiting HDAC3 to the Runx2 promoter to impede Runx2 expression PMID: 27864346
5. Cbx4 is an independent prognostic factor for HCC patients, and the patients with high Cbx4 expression should receive postoperative TACE treatment to improve their survival PMID: 25766328
6. our study demonstrated that miR-195 acts as a tumor suppressor by directly targeting CBX4 in hepatocellular carcinoma PMID: 25607636
7. Role of Cbx4 in migration and metastasis of a metastatic hepatocellular carcinoma cell line. Results show that Cbx4 overexpression increases the in vitro vessel formation of vascular endothelial cells in its SUMO interaction motifs-dependent manner. PMID: 24838576
8. The presence of CBX4-GFP in the same focus had a minor impact on BMI1 and RING1 recovery kinetics. PMID: 24460908
9. High cytoplasmic expression of CBX4 is associated with hepatocellular carcinoma. PMID: 23943028
10. our findings demonstrate that Cbx4 plays a critical role in tumor angiogenesis by governing HIF-1alpha protein PMID: 24434214
11. ASXL1 associates with the PRC2 and loss of ASXL1 in vivo collaborates with NRASG12D to promote myeloid leukemogenesis. PMID: 22897849
12. High Polycomb repressive complex protein is associated with mesothelioma. PMID: 22028491
13. Pc2, methylation controls the protein's interaction with two distinct ncRNAs, TUG1 and NEAT2, which results in the exclusive subnuclear localization of methylated and unmethylated Pc2 in Polycomb bodies and interchromatin granules, respectively. PMID: 22078878
14. plays role in regulation of epidermal stem cell proliferation and senescence PMID: 21885019
15. Data show that PRC2 can integrate information provided by pre-existing histone modifications to accurately tune its enzymatic activity within a particular chromatin context. PMID: 21549310
16. the hPc2-induced SUMOylation of alpha-synuclein could function as a cytoprotector by increasing alpha-synuclein aggregate formation within fibroblast cells. PMID: 21256122
17. 14-3-3 protein binding to chromobox homolog 4 (CBX4) appears to modulate the interaction between CBX4 and the BMI1/PCGF components of polycomb recessive complex 1, but has no effect on CBX4-RING1/RNF2 interaction PMID: 21282530
18. A phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. PMID: 19336002
19. Huntingtin is a novel stimulator of polycomb repressive complex 2. PMID: 19933700
20. Pc2 dramatically enhances CtBP sumoylation. Pc2 is a SUMO E3, and Polycomb Group bodies may be sumoylation centers. PMID: 12679040
21. polycomb group (PcG) protein HPC2, which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins PMID: 15710417
22. SIP1 sumoylation by Pc2 attenuates transcriptional repression of E-cadherin PMID: 16061479
23. Pc2 binds to and colocalizes with homeodomain interacting protein kinase 2 (HIPK2) and serves as a SUMO E3 ligase for this kinase. PMID: 17018294
24. The Endoplasmic reticulum-associated degradation (ERAD) machinery may thus be important for autosomal dominant polycystic kidney disease pathogenesis because the regulation of PC2 expression by the ERAD pathway is altered by mutations in PC2. PMID: 18178578
25. Results show that DNA methyltransferase 3B enhances polycomb protein 2-mediated transcriptional repression of FGFR3, and suggest that DNMT3B is a co-repressor of hPc2 in inducing transcriptional repression independent of DNA methylation. PMID: 18567530
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亞細胞定位：

Nucleus. Nucleus speckle.
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 1554

OMIM: 603079

KEGG: hsa:8535

STRING: 9606.ENSP00000269397

UniGene: Hs.405046

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