ENO2 Monoclonal Antibody

說明書

中文名稱：

ENO2鼠單克隆抗體
貨號：

CSB-MA000224
規(guī)格：

￥1090
圖片：
IHC staining of Human small cell carcinoma of lung tissue, diluted at 1:200.

Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates, diluted at 1:3000.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

本產(chǎn)品為高特異性ENO2（α-烯醇化酶）單克隆抗體，靶向人源、小鼠及大鼠物種的ENO2蛋白，適用于ELISA、Western Blot及免疫組化（IHC）實驗。ENO2作為糖酵解途徑關(guān)鍵酶，在神經(jīng)內(nèi)分泌腫瘤、膠質(zhì)瘤等疾病研究中具有重要價值，其異常表達(dá)與能量代謝調(diào)控及細(xì)胞增殖密切相關(guān)。該抗體經(jīng)嚴(yán)格驗證，在多種樣本體系中展現(xiàn)優(yōu)異的結(jié)合特異性，可精準(zhǔn)識別約47kDa的天然及重組ENO2蛋白，適用于腦組織、腫瘤細(xì)胞等樣本的蛋白定位與定量分析。科研人員可將其應(yīng)用于神經(jīng)退行性疾病機制研究、腫瘤代謝組學(xué)分析及分子病理學(xué)探索，為疾病生物標(biāo)志物篩選、信號通路解析提供可靠工具，滿足神經(jīng)科學(xué)、癌癥研究等領(lǐng)域的多元化科研需求。
Uniprot No.：

P09104
基因名：

ENO2
別名：

2 phospho D glycerate hydrolyase antibody; 2-phospho-D-glycerate hydro-lyase antibody; Eno 2 antibody; ENO2 antibody; ENOG antibody; ENOG_HUMAN antibody; Enolase 2 (gamma; neuronal) antibody; Enolase 2 antibody; Enolase 2 gamma neuronal antibody; Enolase2 antibody; Epididymis secretory protein Li 279 antibody; Gamma enolase antibody; Gamma-enolase antibody; HEL S 279 antibody; Neural enolase antibody; Neuron specific enolase antibody; Neuron specific gamma enolase antibody; Neuron-specific enolase antibody; neuronal enriched enolase antibody; Neurone specific enolase antibody; NSE antibody
宿主：

Mouse
反應(yīng)種屬：

Human,Mouse,Rat
免疫原：

Synthetic Peptide
免疫原種屬：

H, M, R
標(biāo)記方式：

Non-conjugated
抗體亞型：

IgG
克隆號：

5C7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
基因功能參考文獻：
1. CgA and NSE are clinically valuable tumor markers in neuroblastoma and they merit prospective clinical evaluations as such. PMID: 29737901
2. the major result was that elevated serum NSE appears to correlate with prognosis in advanced prostate cancer (PCa), particularly in patients with progressive and metastatic castration-resistant PCa. Based on the existing literature, the role of serum NSE in PCa patients should be further evaluated. PMID: 28885659
3. Identify a possible cross-reactive conformational B cell epitope, shared by Borrelia enolase and gamma enolase, but not by alpha enolase. We hypothesize that this autoantibody interferes with glycolysis in neuronal cells, as the physiological basis for chronic symptoms in at least some cases of post-treatment Lyme disease syndrome. PMID: 29317049
4. We investigated the possible correlation between TCD results and S100B, NSE and LDH levels as well as between NSE-LDH and S100B-LDH levels by regression analysis. We found a statistically significant relationship for both NSE, S100B with LDH. PMID: 29244749
5. ENO2 promoted cell growth, glycolysis, and glucocorticoid resistance, all of which were effectively inhibited when ENO2 was silenced with shRNAs and up-regulated various glycolysis-related genes and enhanced Akt activity with subsequent glycogen synthase kinase3beta (GSK-3beta) phosphorylation, inducing cell proliferation and glycolysis. PMID: 29689546
6. High Neuron-Specific Enolase expression is associated with poor response to therapy in small cell lung cancer. PMID: 28127669
7. In neonates, NSE and s100B levels increase after bypass surgery and return below preoperative baseline levels by postoperative day seven. PMID: 28571730
8. elevated preoperative NSE serum level is adverse predictive and prognostic indicator in adenosquamous lung carcinoma PMID: 27623437
9. There is a significant correlation between mortality in the critically ill patients in the intensive care unit and increased serum concentration of S100B and NSE. PMID: 29222857
10. The detection of TK1 combined with that of cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
11. Serum levels of neuron-specific enolase (and S100B) are both significantly increased postoperatively and predict postoperative cognitive dysfunction. PMID: 27433840
12. This study indicates that serum neuron-specific enolase is elevated in diabetic ketoacidosis and correlated with the severity of hyperglycemia, ketosis, and acidosis. PMID: 28056586
13. serum level elevated in small cell lung cancer patients with shorter progression-free survival PMID: 29145241
14. Levels of NSE and S100B are still elevated 1 year postpartum in women who have had preeclampsia in contrast to women with previous normal pregnancies. PMID: 27653032
15. High NSE expression is associated with hypoxic-ischemic encephalopathy. PMID: 28931055
16. we conducted a retrospective study to evaluate the correlation between clinical-pathological parameters, serum levels of neuron-specific enolase and CYFRA21-1, and bone metastases in lung cancer patients.Multivariate logistic regression analysis showed that patients' age concentrations of neuron-specific enolase and histopathological types were the independent risk factors for bone metastases in patients with lung cancer PMID: 28671048
17. Suggest that NSE may be a practical parameter that can be used to monitor TB activity and treatment response. Elevated serum NSE level originates, at least in part, from macrophages in granulomatous lesions. PMID: 27271274
18. HER2 and NSE serum levels were independent prognostic factors in patients with brain metastases in breast cancer. Serum HER2 and MMP-9 appear to be associated with BM in metastatic BC patients. Their predictive value for BM still needs to be evaluated in further prospective studies. PMID: 27464303
19. this study shows that neuron-specific enolase level is both a predictive marker of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors treatment and a prognostic marker in non-small cell lung cancer harboring EGFR mutations patients PMID: 26719155
20. The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
21. High neuron-specific enolase expression is associated with poor response to chemotherapy in small cell lung cancer. PMID: 26886220
22. In pregnant women developing preeclampsia, the levels of NSE remained high throughout pregnancy, compared to healthy women where the levels decline. PMID: 26355117
23. Serum neuron specific enolase was a useful marker in predicting subclinical brain damage following essential hypertension. PMID: 27000243
24. BALF and serum NSE, CEA, and CYFRA21.1 elevated in lung cancer, which had prompt value for pathology, especially significant for BALF. PMID: 27072263
25. Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing non-small cell lung cancer (NSCLC) patients who depended on the currently limited biomarker development. PMID: 27072222
26. higher serum and nasal secretion levels were measured in patients with chronic rhinosinusitis compared with healthy controls PMID: 26867533
27. CEA, NSE, CA125 and pro-GRP could serve as biomarkers for SCLC, and CEA and CYFRA21-1 could serve as biomarkers for NSCLC. Pro-GRP, CA125 and CEA were related to the clinical stages of lung cancer PMID: 26560853
28. Serum neuron-specific enolase level is an independent predictor of overall survival in patients with gastroenteropancreatic neuroendocrine tumors PMID: 26712902
29. Vimentin, Nestin and WT1. Sox2 was expressed by the stem/progenitor cells of the ventricular zone, whereas the postmitotic neurons of the cortical plate were immunostained by PAX2 and NSE. PMID: 26972711
30. Elevation of neuron specific enolase and brain iron deposition is associated with beta-propeller protein-associated neurodegeneration. PMID: 26481852
31. Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
32. Neuron specific enolase levels were not effective in predicting outcomes or detecting recurrences of Merkel cell carcinoma. PMID: 26299616
33. This study inquires into the physiological concentrations of NSE and S100B proteins in CSF, carried out on a sufficiently large group of 601 patients. PMID: 26421286
34. NSE values is the best predictors of poor 6-month outcome after out-of-hospital cardiac arrest PMID: 26324761
35. Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury. PMID: 25341992
36. The association was studied of intracranial pressure (ICP) and cerebral perfusion pressure (CPP) on S-100B and neuron-specific enolase (NSE) in severe traumatic brain injury (sTBI). PMID: 25518864
37. In this study, the appearance of NeuN-positive cells were observed in the basal plate as early as 5 month of gestation. PMID: 25904356
38. the present findings emphasize that S100 and NSE-immunopositivity might be useful for detecting the cause and process of death due to traumatic brain injury. PMID: 25211554
39. Report ontogenic development of nerve fibers in human fetal livers using immunohistochemical detection of NCAM1/neurone-specific enolase expression. PMID: 25326085
40. The use of serum NSE as a diagnostic marker for disability progression in multiple sclerosis was not supported. PMID: 25686504
41. Serum neuron specific enolase levels were found significantly increased in acute stroke cases as compared to controls. PMID: 24838548
42. Neuron-specific enolase levels were able to diagnose sepsis-associated encephalopathy but with less sensitivity than S100Beta. PMID: 24760429
43. results suggested the use of serum S-100beta and NSE levels as indicators for brain injury PMID: 26040109
44. High levels of neuron-specific enolase (NSE), carbohydrate antigen 125 (CA125) and squamous cell carcinoma antigen (SCC) were detected in 306 (63.6%), 89 (18.5%) and 125 (26.0%) non-small cell lung cancer patients, respectively. PMID: 23712355
45. The role of S100B protein, neuron-specific enolase, and glial fibrillary acidic protein in the evaluation of hypoxic brain injury in acute carbon monoxide poisoning PMID: 24505052
46. NSE expression is a viable biomarker for MM and can be a useful reference for the design and adjustment of clinical MM treatment programs. PMID: 24796851
47. Serial NSE analysis during the acute period of ischemic stroke is useful for monitoring hemorrhagic transformation and the blood-brain barrier disruption status PMID: 25183561
48. Serum NSE levels are closely associated with peripheral neuropathy in patients with diabetes. PMID: 23846809
49. This is the first study to suggest blood NFL mRNA as a surrogate marker for early prediction of prediabetic peripheral neuropathy, while NSE mRNA levels may be of no diagnostic value in prediabetic patients. PMID: 24733614
50. In patients treated with mild hypothermia after out of hospital cardiac arrest changes in NSE are more suitable than its absolute serum levels for the prediction of poor neurologic outcome. PMID: 23836424
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亞細(xì)胞定位：

Cytoplasm. Cell membrane.
蛋白家族：

Enolase family
組織特異性：

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
數(shù)據(jù)庫鏈接：

HGNC: 3353

OMIM: 131360

KEGG: hsa:2026

STRING: 9606.ENSP00000229277

UniGene: Hs.511915

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