Rat neurotensin (NTS) ELISA kit

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產品詳情

產品描述：

大鼠神經降壓素(NTS)酶聯免疫試劑盒（CSB-EL016136RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NTS含量。大鼠神經降壓素（NTS）是一種神經肽，在中樞神經系統中發揮重要作用，參與調節進食、快感、血壓等生理過程。其作用機制涉及與特定受體結合，影響神經元活動，進而調節相關生理功能。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于探究神經系統功能、能量代謝調控、腸道生理機制及應激相關疾病模型的分子機制研究，可滿足體外培養細胞、動物組織或體液樣本的檢測需求，為科研工作者提供穩定可靠的數據支持，助力神經生物學、藥理學及基礎醫學領域的探索性研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NtsNeurotensin/neuromedin N [Cleaved into: Large neuromedin N ELISA Kit; NmN-125); Neuromedin N ELISA Kit; NN ELISA Kit; NmN); Neurotensin ELISA Kit; NT); Tail peptide] ELISA Kit
縮寫：

NTS
Uniprot No.：

P20068
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-99
1:8	Average %	89
	Range %	85-93

回收率：

The recovery of rat NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	100	95-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.
亞細胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Packaged within secretory vesicles.
蛋白家族：

Neurotensin family
數據庫鏈接：

STRING: 10116.ENSRNOP00000005706

UniGene: Rn.60814