Rat leukemia inhibitory factor,LIF ELISA Kit

Rat leukemia inhibitory factor,LIF ELISA Kit

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中文名稱：

大鼠白血病抑制因子(LIF)酶聯(lián)免疫試劑盒
貨號：

CSB-E07432r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
促銷：
其他：

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產(chǎn)品描述：

大鼠白血病抑制因子(LIF)酶聯(lián)免疫試劑盒（CSB-E07432r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、支氣管肺泡灌洗液、組織勻漿樣本中的LIF含量。LIF（白血病抑制因子）是一種多效性細胞因子，屬于IL-6家族，參與細胞生長、分化和免疫反應(yīng)。其研究機制涉及調(diào)節(jié)細胞增殖、分化和表型，影響骨代謝、神經(jīng)發(fā)育等。LIF通過與其受體結(jié)合，激活JAK/STAT和MAPK信號通路，參與多種疾病的發(fā)病過程。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于呼吸系統(tǒng)疾病模型研究、干細胞分化調(diào)控實驗及炎癥相關(guān)機制探索，可為肺纖維化、急性肺損傷等動物模型中的LIF動態(tài)監(jiān)測提供可靠工具，助力科研人員深入解析LIF在組織再生、免疫微環(huán)境調(diào)節(jié)中的生物學(xué)功能。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
縮寫：

LIF
Uniprot No.：

P17777
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	93
	Range %	89-97
1:4	Average %	99
	Range %	93-104
1:8	Average %	90
	Range %	86-98

回收率：

The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-92
EDTA plasma (n=4)	95	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.249	2.174	2.212	2.039
10	1.583	1.561	1.572	1.399
5	1.012	0.952	0.982	0.809
2.5	0.599	0.583	0.591	0.418
1.25	0.446	0.416	0.431	0.258
0.625	0.320	0.299	0.310	0.137
0.312	0.258	0.245	0.252	0.079
0	0.177	0.169	0.173

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Menstrual blood-derived mesenchymal stem cells pretreated by low-intensity pulsed ultrasound repair endometrial injury by inhibiting the PI3K/AKT pathway S Huang, C Cheng, Z Wang, R Li, W Li, K Yu,Reproductive BioMedicine Online,2024
3D Bioprinting a human iPSC-derived MSC-loaded scaffold for repair of the uterine endometrium W Ji,Acta Biomaterialia,2020
Effect of irisin on endometrial receptivity of rats with polycystic ovary syndrome Li C, et al,Gynecological Endocrinology,2019
Discovery of Enhancers of the Secretion of Leukemia Inhibitory Factor for the Treatment of Multiple Sclerosis Vela L.et al,J Biomol Screen.,2016

功能：

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
亞細胞定位：

Secreted.
蛋白家族：

LIF/OSM family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000009313

UniGene: Rn.44379

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.