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中文名稱：大鼠腦啡肽(ENK)酶聯免疫試劑盒
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貨號：CSB-E13325r
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規格：96T/48T
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價格：￥3800/￥2500
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促銷：
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其他：
產品詳情
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產品描述：大鼠腦啡肽(ENK)酶聯免疫試劑盒（CSB-E13325r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ENK含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產品適用于神經科學、藥理學等領域的基礎研究，例如探究疼痛機制模型、應激反應調控或藥物干預對ENK表達的影響，為神經系統功能研究提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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縮寫：ENK
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma
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檢測范圍：0.625 ng/mL-40 ng/mL
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靈敏度：0.156 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Neuroscience
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 106 Range % 103-111 1:2 Average % 95 Range % 92-98 1:4 Average % 90 Range % 86-95 1:8 Average % 93 Range % 90-96 -
回收率：
The recovery of rat ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 103 99-106 EDTA plasma (n=4) 91 85-95 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 40 2.347 2.428 2.388 2.291 20 1.651 1.740 1.696 1.599 10 1.344 1.239 1.292 1.195 5 0.837 0.900 0.869 0.772 2.5 0.485 0.477 0.481 0.384 1.25 0.309 0.289 0.299 0.202 0.625 0.200 0.205 0.203 0.106 0 0.095 0.098 0.097 -
數據處理：
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貨期：3-5 working days
引用文獻
- Sucrose and naltrexone prevent increased pain sensitivity and impaired long-term memory induced by repetitive neonatal noxious stimulation: Role of BDNF and β-endorphin Nuseir KQ.et al,Physiol Behav.,2017
- Sucrose-induced analgesia during early life modulates adulthood learning and memory formation Khawla Q. Nuseir et al,Physiology & Behavior,2015
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