Rat Interleukin 11,IL-11 ELISA KIT

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠白介素11(IL-11)酶聯(lián)免疫試劑盒（CSB-E07358r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL11含量。IL11是重要靶點。它是一種細胞因子，參與多種生理和病理過程。在研究機制上，IL11與受體結(jié)合激活信號通路，影響細胞的增殖、分化等。當(dāng)前圍繞它在腫瘤、纖維化疾病等方面的研究較多，有望為相關(guān)疾病治療帶來新策略。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，科研人員可借助該產(chǎn)品探究IL - 11在炎癥性疾病模型、再生醫(yī)學(xué)研究或藥物干預(yù)實驗中的動態(tài)變化，為揭示其生物學(xué)功能及信號通路機制提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Il11 ELISA Kit; Interleukin-11 ELISA Kit; IL-11 ELISA Kit
縮寫：

IL11
Uniprot No.：

Q99MF5
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-94
1:2	Average %	85
	Range %	80-90
1:4	Average %	92
	Range %	86-101
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	80-105
EDTA plasma (n=4)	98	90-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'.
亞細胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000023489

UniGene: Rn.198483