Rat Fibronectin,FN ELISA Kit

Rat Fibronectin,FN ELISA Kit

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中文名稱：

大鼠纖連蛋白(FN)酶聯免疫試劑盒
貨號：

CSB-E04553r
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產品描述：

大鼠纖連蛋白(FN)酶聯免疫試劑盒（CSB-E04553r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的FN1含量。FN1，即纖連蛋白1，是一種存在于血漿和細胞外基質中的糖蛋白，參與細胞粘附和遷移等過程。研究顯示，FN1在運動中通過激活肝臟自噬和改善胰島素敏感性來促進機體健康，其具體機制涉及骨骼肌分泌FN1與肝臟α5β1整合素受體結合，進而調節肝臟自噬和胰島素敏感性。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于體外培養細胞分泌功能評估、組織工程模型基質成分分析，以及心血管疾病、纖維化疾病等動物模型中細胞外基質動態變化的科研探索，助力于細胞行為機制、病理生理過程及相關藥物篩選等基礎研究領域的數據獲取。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
縮寫：

FN1
Uniprot No.：

P04937
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	85-100
1:2000	Average %	87
	Range %	82-93
1:4000	Average %	98
	Range %	95-100
1:8000	Average %	92
	Range %	88-94

回收率：

The recovery of rat FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	99	89-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.370	2.405	2.388	2.245
25	2.015	2.063	2.039	1.896
12.5	1.613	1.627	1.620	1.477
6.25	1.115	1.136	1.126	0.983
3.12	0.685	0.679	0.682	0.539
1.56	0.432	0.467	0.450	0.307
0.78	0.278	0.291	0.285	0.142
0	0.142	0.144	0.143

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
數據庫鏈接：

STRING: 10116.ENSRNOP00000019772

UniGene: Rn.1604

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.