Rat Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠脫氫表雄酮硫酸酯(DHEA-S)酶聯(lián)免疫試劑盒（CSB-E05106r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的DHEA-S含量。DHEA-S即硫酸脫氫表雄酮，是腎上腺皮質(zhì)分泌的一種激素前體。它在人體生理活動(dòng)中起重要作用，如影響代謝、免疫等。研究機(jī)制上，其可轉(zhuǎn)化為雄激素和雌激素，參與調(diào)節(jié)多種生理功能，對它的研究有助于深入了解人體內(nèi)分泌及相關(guān)疾病機(jī)制。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品適用于動(dòng)物模型中的激素水平監(jiān)測、代謝性疾病機(jī)制研究、應(yīng)激生理學(xué)分析等科研場景，例如通過檢測大鼠血清/血漿DHEA - S濃度，探究腎上腺皮質(zhì)功能狀態(tài)或激素相關(guān)疾病的分子調(diào)控網(wǎng)絡(luò)，為內(nèi)分泌領(lǐng)域的機(jī)制研究提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

DHEA-S
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	81-92
1:200	Average %	96
	Range %	90-100
1:400	Average %	95
	Range %	91-104
1:800	Average %	92
	Range %	84-98

回收率：

The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	97	91-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.