Rat Cytochrome c(CYCS) ELISA kit

Rat Cytochrome c(CYCS) ELISA kit

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文獻引用 (19) 說明書下載

中文名稱：

大鼠細胞色素C(CYCS)酶聯免疫試劑盒
貨號：

CSB-EL006328RA
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

大鼠細胞色素C(CYCS)酶聯免疫試劑盒（CSB-EL006328RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYCS含量。CYCS 即細胞色素 c，是位于線粒體內膜外側的水溶性蛋白。它在細胞呼吸的電子傳遞鏈中發揮關鍵作用。研究發現，其從線粒體釋放到細胞質會激活凋亡相關蛋白，進而啟動細胞凋亡機制，在細胞死亡調控等研究領域備受關注。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于大鼠模型中CYCS相關的基礎研究，例如線粒體功能評估、凋亡信號通路探索、藥物誘導的細胞損傷研究或心血管疾病模型的分子機制分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CycsCytochrome c ELISA Kit; somatic ELISA Kit
縮寫：

CYCS
Uniprot No.：

P62898
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	89
	Range %	85-96
1:4	Average %	92
	Range %	90-95
1:8	Average %	91
	Range %	87-96

回收率：

The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	90	84-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.581	2.458	2.520	2.427
2500	2.234	2.186	2.210	2.117
1250	1.638	1.621	1.630	1.537
625	1.103	1.097	1.100	1.007
312	0.692	0.672	0.682	0.589
156	0.393	0.386	0.390	0.297
78	0.236	0.223	0.230	0.137
0	0.094	0.092	0.093

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
亞細胞定位：

Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
蛋白家族：

Cytochrome c family
組織特異性：

Found in embryos and in adult liver and heart.
數據庫鏈接：

KEGG: rno:100363502

STRING: 10116.ENSRNOP00000041521

UniGene: Rn.161841

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.