Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit
-
中文名稱：大鼠Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒
-
貨號(hào)：CSB-E08092r
-
規(guī)格：96T/48T
-
價(jià)格：￥3900/￥2500
-
促銷：
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：大鼠Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒（CSB-E08092r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Col Ⅱ含量。Col Ⅱ即Ⅱ型膠原，是軟骨基質(zhì)的主要成分。其與關(guān)節(jié)炎等疾病密切相關(guān)，自身抗體識(shí)別可觸發(fā)免疫反應(yīng)。研究機(jī)制主要圍繞其在軟骨穩(wěn)態(tài)維持、免疫調(diào)節(jié)中的作用，探索其在疾病診斷、治療靶點(diǎn)方面的價(jià)值。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于軟骨發(fā)育、退行性關(guān)節(jié)疾病機(jī)制研究、藥物或生物材料對(duì)軟骨代謝影響的體外評(píng)估等科研領(lǐng)域，為探索Col Ⅱ在骨骼系統(tǒng)相關(guān)疾病中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
-
別名：Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
-
縮寫：Col Ⅱ
-
種屬：Rattus norvegicus (Rat)
-
樣本類型：serum, plasma, cell culture supernates, tissue homogenates
-
檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
-
靈敏度：3.9 pg/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測(cè)波長(zhǎng)：450 nm
-
研究領(lǐng)域：Signal Transduction
-
測(cè)定原理：quantitative
-
測(cè)定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 87 Range % 83-92 1:200 Average % 94 Range % 90-99 1:400 Average % 92 Range % 88-96 1:800 Average % 102 Range % 98-105 -
回收率：
The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 93-96 EDTA plasma (n=4) 98 89-104 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.584 2.599 2.592 2.438 500 2.217 2.253 2.235 2.081 250 1.765 1.792 1.779 1.625 125 1.292 1.264 1.278 1.124 62.5 0.789 0.773 0.781 0.627 31.2 0.396 0.388 0.392 0.238 15.6 0.257 0.269 0.263 0.109 0 0.153 0.155 0.154 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- 7-Hydroxycoumarin mitigates the severity of collagen-induced arthritis in rats by inhibiting proliferation and inducing apoptosis of fibroblast-like synoviocytes via suppression of Wnt/β-catenin signaling pathway Li Cai,Phytomedicine: international journal of phytotherapy and phytopharmacology,2021
- Acetazolamide ameliorates the severity of collagen-induced arthritis in rats: Involvement of inducing synovial apoptosis and inhibiting Wnt/β-catenin pathway Y Mu,International immunopharmacology,2020
- miR-16 integrates signal pathways in myofibroblasts: determinant of cell fate necessary for fibrosis resolution Q Pan,Cell Death & Disease,2020
- Novel Long Non-Coding RNA lnc-URIDS Delays Diabetic Wound Healing By Targeting Plod1 M Hu,Diabetes,2020
- The Influence of bFGF on the Fabrication of Microencapsulated Cartilage Cells under Different Shaking Modes Xia Zhou , et al,Polymers,2019
- Jatrorrhizine Hydrochloride Suppresses Proliferation, Migration, and Secretion of Synoviocytes In Vitro and Ameliorates Rat Models of Rheumatoid Arthritis In Vivo Haiwen Qiu.et al,Int. J. Mol. Sci.,2018
- Mechanical stretch promotes matrix metalloproteinase-2 and prolyl-4-hydroxylase a1 production in human aortic smooth muscle cells via Akt-p38 MAPK-JNK signaling. Liu X et al,Int J Biochem Cell Biol,2015
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
