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中文名稱：大鼠天門冬氨酸氨基轉移酶(AST)酶聯免疫試劑盒
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貨號：CSB-E13023r
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠天門冬氨酸氨基轉移酶(AST)酶聯免疫試劑盒（CSB-E13023r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GOT1含量。GOT1是一種重要靶點。它參與細胞內氨基酸代謝，在細胞能量生成和生物合成中起關鍵作用。研究發現其異常表達與多種疾病尤其是腫瘤發生發展相關，解析其相關機制可助力開發以GOT1為靶點的新型治療藥物和策略。試劑盒檢測范圍為3.12 mIU/mL-200 mIU/mL，在肝臟病理機制研究、心肌功能實驗及藥物毒性評估等科研場景中具有重要應用價值；適用于體外實驗中的組織損傷模型評估、生化指標動態監測及相關分子機制探索，為疾病模型構建、藥效學評價等基礎研究提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit
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縮寫：GOT1
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma
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檢測范圍：3.12 mIU/mL-200 mIU/mL
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靈敏度：0.78 mIU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 87-92 1:2 Average % 104 Range % 100-107 1:4 Average % 93 Range % 88-97 1:8 Average % 98 Range % 92-103 -
回收率：
The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 95 91-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mIU/ml OD1 OD2 Average Corrected 200 2.094 2.068 2.081 1.918 100 1.276 1.310 1.293 1.130 50 0.838 0.784 0.811 0.648 25 0.521 0.539 0.530 0.367 12.5 0.353 0.334 0.344 0.181 6.25 0.300 0.288 0.294 0.131 3.12 0.241 0.237 0.239 0.076 0 0.164 0.162 0.163 -
數據處理：
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貨期：3-5 working days
引用文獻
- Hepatocyte activation and liver injury following cerebral ischemia promote HMGB1-mediated hepcidin upregulation in hepatocytes and regulation of systemic iron levels D Davaanyam, SI Seol, SA Oh, H Lee,Experimental & Molecular Medicine,2024
- Efficacy and safety of intra-articular-only meropenem after one-stage revision for treating Escherichia coli-induced periprosthetic joint infection in a rat model Y Li, S Wuermanbieke, F Wang, W Mu,Bone & Joint Research,2024
- Effect of Liv.52hb, Ganoderma Lucidum, Stc30, and Astaxanthin on Serum TNF-α And Liver Function Indices Following CCL4 Induced Hepatocellular Carcinoma in Albino Wistar Rats Model JT Johnson, EU Modo,International Journal of Research in Medical and Clinical Science,2024
- Intranasal delivery of sunitinib: A new therapeutic approach for targeting angiogenesis of glioblastoma E Seidkhani,Toxicology and applied pharmacology,2023
- Intra-wound versus systemic vancomycin for preventing surgical site infection induced by methicillin-resistant S. aureus after spinal implant surgery in a rat model J Wei,Journal of orthopaedic surgery and research,2023
- Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis G Angelini,Nature communications,2022
- Co-treatment with grape seed extract and mesenchymal stem cells in vivo regenerated beta cells of islets of Langerhans in pancreas of type I-induced diabetic rats A Farid,Stem cell research & therapy,2022
- The complement C3a?C3aR and C5a?C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF?κB signaling S Luo,Experimental and Therapeutic Medicine,2022
- Cadmium chloride induces non-alcoholic fatty liver disease in rats by stimulating miR-34a/SIRT1/FXR/p53 axis AS Alshehri,Science of The Total Environment,2021
- RNA-sequencing analysis of the effect of luteolin on methamphetamine-induced hepatotoxicity in rats: a preliminary study Qu D,PeerJ,2020
- Lactulose attenuates METH-induced neurotoxicity by alleviating the impaired autophagy, stabilizing the perturbed antioxidant system and suppressing apoptosis in rat striatum Xie XL.et al,Toxicology Letters Volume 289, 1 June 2018, Pages 10,2018
- Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies Wang Q.et al,Food Chem Toxicol. ,2017
- Different roles of hepatic hypothermic ischemia and ischemic preconditioning in chemically induced hepatocarcinogenesis in rats Qingan Qi et al,Journal of Surgical Research,2014
- Consumption of Oxidized Soybean Oil Increased Intestinal Oxidative Stress and Affected Intestinal Immune Variables in Yellow-feathered Broilers Fangfang Liang. et al,Asian Australas. J. Anim. Sci. ,2015
- Ameliorative potential of stem bromelain on lead-induced toxicity in Wistar rats Al-Otaibi WR. et al,Acta Biol Hung,2015
產品評價
相關問答
■ 常見問題解答
Q:
Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since we cannot collect more than 150 μL of blood from mice, we can use only 50 μL serum for ELISA. Will it fall within the detectable range ?
A:
We are glad to inform you the following details:
CSB-E13023r,Rat Aspartate Aminotransferase (AST) ELISA kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
If any further questions,pls let me know.
CSB-E13023r,Rat Aspartate Aminotransferase (AST) ELISA kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
If any further questions,pls let me know.
靶點詳情
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功能：Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
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基因功能參考文獻：
- Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, mercaptopyruvate sulfurtransferase (MPST)and cysteine aminotransferase (CAT). PMID: 24611772
- In vivo lentiviral gene delivery of GOT minimized lesion volume, whereas GOT knockdown worsened stroke outcomes. Thus, brain tissue GOT emerges as a novel target in managing stroke outcomes. PMID: 21361730
- Results suggest that downregulation of the endogenous SO(2)/GOT pathway is likely involved in the pathogenesis of ISO-induced myocardial injury. PMID: 20733562
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亞細胞定位：Cytoplasm.
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蛋白家族：Class-I pyridoxal-phosphate-dependent aminotransferase family
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組織特異性：Expressed in liver and kidney.
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數據庫鏈接：
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