Rabbit Interleukin 1β,IL-1β ELISA Kit

Rabbit Interleukin 1β,IL-1β ELISA Kit

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文獻引用 (14) 說明書下載

中文名稱：

兔子白介素1β (IL-1β)酶聯免疫試劑盒
貨號：

CSB-E06900Rb
規格：

96T/48T
價格：

￥3800/￥2500
促銷：
其他：

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產品描述：

兔子白介素1β (IL-1β)酶聯免疫試劑盒（CSB-E06900Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B即白介素-1β，是一種炎癥細胞因子，在炎癥反應、免疫調節等生理病理過程中扮演重要角色。研究表明，它能激活下游信號通路，介導炎性反應。異常表達與多種疾病如關節炎、心血管疾病等密切相關，是藥物研發的熱門靶點。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外實驗中對動物模型（如關節炎、膿毒癥等疾病模型）的炎癥機制研究、藥物干預效果評估或免疫調節通路分析；為科研工作者在基礎醫學、藥理學及免疫學領域提供靈敏穩定的檢測工具，支持細胞因子網絡調控的深入探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
縮寫：

IL1B
Uniprot No.：

P14628
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	85-105
1:2	Average %	95
	Range %	85-103
1:4	Average %	94
	Range %	82-100
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	82-104
EDTA plasma (n=4)	95	85-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.852	2.713	2.783	2.674
250	2.235	2.245	2.240	2.131
125	1.424	1.392	1.408	1.299
62.5	0.753	0.784	0.769	0.660
31.2	0.447	0.433	0.440	0.331
15.6	0.266	0.282	0.274	0.165
7.8	0.202	0.195	0.199	0.090
0	0.111	0.106	0.109

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The effect of dietary eugenol nano-emulsion supplementation on growth performance, serum metabolites, redox homeostasis, immunity, and pro-inflammatory responses of growing rabbits under heat stress SS Alrashedi,Open veterinary journal,2024
Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis T Liu,Neural regeneration research,2024
Use of the improved tug-of-war acupuncture for promoting cartilage repair by inducing macrophage polarization in knee osteoarthritis P Tangseefa,Heliyon,2024
Skin fibroblasts' alteration after Acupuncture on LI11 in Rabbits with bacteria endotoxin induced fever W Fan,Acupuncture & Electro-Therapeutics Research,2021
Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural Staphylococcus aureus skin infections A Mu帽oz-Silvestre,The American Journal of Pathology,2020
Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits H Zhao,czech journal of animal science,2020
Effect of febuxostat on biochemical parameters of hyperlipidemia induced by a high-fat diet in rabbits Heikal MM,Canadian Journal of Physiology and Pharmacology,2019
Evaluation of comparative soft tissue response to bone void fillers with antibiotics in a rabbit intramuscular model Oliver RA, et al,Journal of Biomaterials Applications,2019
Effect of febuxostat on biochemical parameters of high fat diet-induced hyperlipidemia in rabbits Heikal M, et al,Canadian journal of physiology and pharmacology,2019
In Vitro osteoarthritic model to evaluate the regenerative capability of tissue engineered constructs and bioactive implants for cartilage regeneration Neethu Mohan .et al,/,2017
Experimental Study of Phlebitis Ointment Administration in Acute Superficial Thrombophlebitis Guangzong Li.et al,EVID-BASED COMPL ALT,2018
Involvement of AMPK/SIRT1 pathway in anti-allodynic effect of troxerutin in CCI-induced neuropathic pain Gui Y. et al,Eur J Pharmacol,2015
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue Oliver RA.et al,PLoS One.,2015
Assessing the Systemic Toxicity in Rabbits after Sub Acute Exposure to Ocular Irritant Chemicals Reshma CS et al,Toxicol Res,2015

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功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
基因功能參考文獻：
1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
2. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
3. IL-1beta induced a significant reduction in the relative intrinsic activity of GLUT5 and in this decrease are involved NO signal pathways; blockage of D-fructose intestinal uptake by IL-1beta may play an essential role in the pathophysiology of septic shock. PMID: 20803526
4. The inhibitory effect of IL-1beta on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor kappa B. PMID: 22759965
5. Glucosamine hydrochloride treatment can can partially decrease the expression levels of IL-1 beta and increase the expression levels of TGF-beta 1, which delays the development of osteoarthritis. PMID: 20369526
6. In this study evidence is provided that exogenous PGF2alpha differentially modulates luteal expression of IL1B transcripts depending on luteal stage. PMID: 20427144
7. results revealed that a transient episode of raised-intensity phonation causes a significant increase in vocal fold inflammatory mRNA expression - IL-1beta,COX-2, and TGFbeta1 PMID: 20879195
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亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

KEGG: ocu:100008990

STRING: 9986.ENSOCUP00000021869

UniGene: Ocu.1993

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.