Rabbit Epidermal growth factor,EGF ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子表皮生長因子(EGF)酶聯免疫試劑盒（CSB-E06909Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGF含量。表皮生長因子（EGF）是一種重要的生長因子，屬于表皮生長因子家族，通過與其受體EGFR結合，激活細胞內的信號傳導途徑，促進細胞增殖、分化、遷移和存活。EGF在胚胎發育、組織再生和傷口愈合中發揮關鍵作用，同時其異常表達與多種疾病，尤其是癌癥的發生發展密切相關。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL，特別適用于探究EGF在皮膚屏障功能、消化道黏膜修復、創傷愈合等研究領域的調控機制。科研人員可利用分析不同生理/病理條件下體液和組織中的EGF動態變化，為研究生長因子在再生醫學、腫瘤微環境中的作用提供量化依據，其寬檢測范圍設計可有效應對復雜生物樣本中目標蛋白濃度的顯著波動。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

EGF
Uniprot No.：

G1TBJ6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.5 ng/mL-8 ng/mL
靈敏度：

0.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	100-115
1:2	Average %	92
	Range %	84-97
1:4	Average %	100
	Range %	90-104
1:8	Average %	100
	Range %	93-104

回收率：

The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	87-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.