Rabbit Complement fragment 3a,C3a ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 評價 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子補體片斷3a(C3a)酶聯(lián)免疫試劑盒（CSB-E09283Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3a含量。C3a 是補體系統(tǒng)激活產(chǎn)生的重要過敏毒素。它參與炎癥反應(yīng)、免疫調(diào)節(jié)等生理病理過程。研究機制方面，其與 C3a 受體結(jié)合，能介導(dǎo)細胞活化、趨化等。在疾病中過度激活可能引發(fā)不良免疫反應(yīng)，相關(guān)研究或為疾病治療提供新靶點。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于兔源實驗?zāi)Ｐ停ㄈ鐢⊙Y、自身免疫疾病或組織損傷模型）中補體系統(tǒng)激活程度的評估，可幫助研究者分析炎癥反應(yīng)機制、藥物干預(yù)效果或病理狀態(tài)下C3a的動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C3a
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	80-100
1:2	Average %	96
	Range %	85-104
1:4	Average %	97
	Range %	92-104
1:8	Average %	90
	Range %	82-98

回收率：

The recovery of rabbit C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-107
EDTA plasma (n=4)	96	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 個評價

樣品類型：血清

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評價：我用CSB-E09283Rb檢測正常兔血清中β-TG含量，標(biāo)準(zhǔn)曲線的R2可以做到高達0.9997，標(biāo)準(zhǔn)曲線符合要求，樣品OD值其中之一0.301、0.306，可以做到平行，檢測結(jié)果準(zhǔn)確且完美。

By 王老師

樣品類型：血清

樣品信息：其他兔

稀釋比：沒有稀釋

產(chǎn)品評價：我用CSB-E09283RI檢測正常血清，標(biāo)準(zhǔn)曲線的OD值范圍為1.6xx-0.05x，樣本的OD值范圍為0.3-0.4，試劑盒的標(biāo)準(zhǔn)曲線r2>0.99，滿足我們的要求，好評。

By 王老師