Pig basic fibroblast growth factor,bFGF ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬堿性成纖維細胞生長因子(bFGF)酶聯免疫試劑盒（CSB-E12841p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2即成纖維細胞生長因子2，是重要的促血管生成因子。它參與多種生理過程如胚胎發育、組織修復等。研究機制上，FGF2能與細胞膜上受體結合，激活下游信號通路，調控細胞的增殖、分化、遷移等，在腫瘤血管生成等疾病中作用受關注。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，本試劑盒適用于體外研究，如探究豬模型中創傷愈合機制、干細胞培養條件優化或疾病相關生長因子通路分析，為心血管疾病、再生醫學及腫瘤生物學等基礎研究提供可靠的實驗工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
縮寫：

FGF2
Uniprot No.：

Q60487
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.7 pg/mL-300 pg/mL
靈敏度：

1.2 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-100
1:2	Average %	97
	Range %	91-105
1:4	Average %	94
	Range %	90-110
1:8	Average %	95
	Range %	82-105

回收率：

The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-104
EDTA plasma (n=4)	98	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
亞細胞定位：

Secreted. Nucleus.
蛋白家族：

Heparin-binding growth factors family
數據庫鏈接：

STRING: 10141.ENSCPOP00000004847