Pig Epithelial growth factor,EGF ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬表皮生長因子(EGF)酶聯免疫試劑盒（CSB-E06788p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGF含量。EGF（表皮生長因子）是EGF家族的創始成員，具有與家族成員高度相似的結構和功能。EGF是一種低分子量多肽，最初從小鼠頜下腺中純化，后在多種人體組織中發現。EGF通過與細胞表面的EGFR受體結合，刺激細胞增殖、分化和存活，在傷口愈合、組織修復等方面發揮重要作用。試劑盒檢測范圍為250 pg/mL-10000 pg/mL，可廣泛應用于動物營養學、生殖生理學、免疫學等基礎研究領域，例如探究飼料添加劑對腸道黏膜修復的影響、評估應激狀態下EGF的動態變化，或解析特定疾病模型中生長因子調控機制本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

EGF
Uniprot No.：

F1S137
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

250 pg/mL-10000 pg/mL
靈敏度：

125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	93
	Range %	90-96
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	83-90

回收率：

The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	101	99-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.