Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠纖溶酶原激活物抑制因子1(PAI-1)酶聯(lián)免疫試劑盒（CSB-E07947m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINE1含量。SERPINE1是一個(gè)備受關(guān)注的靶點(diǎn)。它在多種生理和病理過程中發(fā)揮重要作用，如參與凝血、細(xì)胞遷移等。研究機(jī)制上，多聚焦于其在腫瘤進(jìn)展、纖維化疾病等中的作用，通過調(diào)控相關(guān)信號(hào)通路來影響疾病發(fā)展，為相關(guān)疾病治療提供潛在方向。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于心血管疾病模型、血栓形成機(jī)制研究以及創(chuàng)傷后組織修復(fù)等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
縮寫：

SERPINE1
Uniprot No.：

P22777
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.22 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	94
	Range %	88-102
1:8	Average %	96
	Range %	91-103

回收率：

The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. Involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Serpin family
數(shù)據(jù)庫鏈接：

STRING: 10090.ENSMUSP00000039586

UniGene: Mm.250422