Mouse oxyntomodulin ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠胃泌酸調節素酶聯免疫試劑盒（CSB-E12950m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的oxyntomodulin含量。小鼠胃泌酸調節素（OXM）是一種由胃內分泌細胞分泌的肽激素，主要作用是調節胃酸分泌和胃腸道功能。其研究機制涉及對胃腸激素、腸道菌群、胃腸道神經遞質、腦-腸肽、Cajal間質細胞和胃腸電活動的調節，以及苦味受體和離子通道的影響。試劑盒檢測范圍為0.312 pg/mL-20 pg/mL，適用于胃腸生理機制、藥物干預效果評估或相關疾病模型的體外研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

oxyntomodulin
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 pg/mL-20 pg/mL
靈敏度：

0.078 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	92-108
1:10	Average %	89
	Range %	85-96
1:20	Average %	97
	Range %	91-105
1:40	Average %	92
	Range %	87-96

回收率：

The recovery of mouse oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.