Mouse interferon alpha-2(IFNA2) ELISA kit

說明書下載

產品詳情

產品描述：

小鼠干擾素α2(IFNA2)酶聯免疫試劑盒（CSB-EL011038MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IFNA2含量。IFNA2 即干擾素α2，是一種細胞因子。它具有抗病毒、抗腫瘤和免疫調節等作用。其研究機制主要在于它能與細胞表面受體結合，激活信號通路，誘導下游多種抗病毒和免疫相關基因表達，從而發揮生物學效應。試劑盒檢測范圍為9.38 pg/mL-600 pg/mL，適用于體外實驗中IFNA2表達水平的動態監測，例如病毒學研究中宿主免疫應答評估、免疫調節機制探索或炎癥相關疾病模型構建等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit
縮寫：

IFNA2
Uniprot No.：

P01573
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

9.38 pg/mL-600 pg/mL
靈敏度：

2.35 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	102
	Range %	98-107
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

回收率：

The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	94-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.