Mouse acetyl-CoA ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠乙酰輔酶A酶聯免疫試劑盒（CSB-E12936m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的acetyl-CoA含量。Acetyl - CoA即乙酰輔酶A，是生物體代謝關鍵物質，在糖、脂肪、蛋白質代謝中起樞紐作用。研究發現，它參與三羧酸循環、脂肪酸合成等多種代謝途徑，其水平變化會影響細胞代謝平衡，也與糖尿病、癌癥等疾病的發生發展可能相關。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于研究能量代謝、脂肪酸合成及細胞調控等機制，特別針對小鼠模型中的乙酰輔酶A代謝通路探索，例如在代謝性疾病、營養干預或藥物作用等科研場景中，可通過多種生物樣本進行定量分析，為體外實驗和離體組織研究提供可靠工具。滿足科研工作者對乙酰輔酶A相關分子機制的高通量研究需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

acetyl-CoA
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	87-93
1:40	Average %	103
	Range %	100-105
1:80	Average %	93
	Range %	87-96
1:160	Average %	98
	Range %	93-101

回收率：

The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.