Human β-defensins 1 ELISA Kit
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中文名稱：
人β-防御素1酶聯免疫試劑盒
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貨號：
CSB-E14186h
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規格：
96T/48T
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價格：
￥3600/￥2500
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其他：
產品詳情
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產品描述：
人β-防御素1酶聯免疫試劑盒（CSB-E14186h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DEFB1含量。DEFB1（防御素β1）是一種抗菌肽，屬于β-防御素家族，由上皮細胞高度表達。其編碼的蛋白質具有抗菌活性，對上皮表面抵抗微生物定植起重要作用。研究顯示，DEFB1在先天免疫反應中發揮關鍵作用，與哮喘、嚴重膿毒癥等疾病的發生機制有關。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本產品適用于科研領域探索hBD - 1在病原體感染模型、炎癥反應機制或組織微環境調控中的動態變化，為免疫調控研究及藥物開發提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
BD 1 ELISA Kit; BD-1 ELISA Kit; BD1 ELISA Kit; beta 1 ELISA Kit; Beta defensin 1 ELISA Kit; Beta-defensin 1 ELISA Kit; DEFB 1 ELISA Kit; DEFB1 ELISA Kit; DEFB1_HUMAN ELISA Kit; DEFB101 ELISA Kit; Defensin ELISA Kit; Defensin beta 1 ELISA Kit; Defensin beta 1 preproprotein ELISA Kit; HBD 1 ELISA Kit; hBD-1 ELISA Kit; HBD1 ELISA Kit; MGC51822 ELISA Kit
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縮寫：
DEFB1
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
3.12 pg/mL-200 pg/mL
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靈敏度：
0.78 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Microbiology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 86 | |
| Range % | 80-90 | |
| 1:10 | Average % | 96 | |
| Range % | 91-100 | |
| 1:20 | Average % | 97 | |
| Range % | 91-105 | |
| 1:40 | Average % | 92 | |
| Range % | 86-98 | |
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回收率：
| The recovery of human β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-99 | |
| EDTA plasma (n=4) | 90 | 85-95 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.544 | 2.520 | 2.532 | 2.412 | | | 100 | 1.879 | 1.760 | 1.820 | 1.700 | | | 50 | 1.165 | 1.084 | 1.125 | 1.005 | | | 25 | 0.678 | 0.639 | 0.659 | 0.539 | | | 12.5 | 0.443 | 0.424 | 0.434 | 0.314 | | | 6.25 | 0.325 | 0.309 | 0.317 | 0.197 | | | 3.12 | 0.198 | 0.189 | 0.194 | 0.074 | | | 0 | 0.123 | 0.117 | 0.120 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled BD-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
產品評價
樣品類型：血漿（抗凝劑） Edta抗凝
樣品信息：人
稀釋比：其他 1:10
產品評價： 我用CSB-E14186h測人外周血血漿HBD水平，od值在0.6-2.0之間，總體上還可以，與以往文獻報道的水平差不多。正常組和患病組的OD值大部分在1.0-1.5之間，有個別是2.0和0.6。稀釋都是1:10稀釋。
靶點詳情
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功能：
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
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基因功能參考文獻：
- The allelic distribution of the polymorphism rs12355840 in miRNA202 was associated with caries experience in the Brazilian Manaus group. In the Ribeirao Preto group, the allelic and genotypic distributions in the polymorphism rs11362 in DEFB1 were associated with caries experience. PMID: 29727722
- These studies identified SNPs that were cis-eQTLs for DEFB1 and AHR and, which were associated with variation in plasma KYN concentrations that were related to severity of major depressive disordersymptoms. PMID: 29317604
- a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006
- identification of critical promoter regions of DEFB1 that are epigenetically influenced by lactobacilli in vaginal keratinocytes PMID: 28508168
- Endometrial tissues and epithelial cells constitutively secreted both BD-1 and -2 mostly at the apical compartment. Both genistein and daidzein induced BDs secretion which reached a peak at 5-15 min. The apical secretion of BDs was coincidence with increased Cl- secretion induced by genistein. PMID: 29905453
- The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05) in children. PMID: 28343232
- These findings suggest a role for hBD-1 in psoriasis pathogenesis PMID: 28067065
- Significant associations were found between periodontitis and g. -20G> A (rs11362) and g. -44C> G (rs1800972) SNPs in DEFB1 gene as well as p.Ala29Thr (rs1126477) and p.Lys47Arg (rs1126478) SNPs in LTF gene. PMID: 28485077
- hBD-1 potentiates the induction of in vitro osteoclastogenesis by RANKL via enhanced phosphorylation of the p44/42 MAPKs. PMID: 28709835
- findings lead us to hypothesize an involvement of hBD-1 mediated innate immunity in the modulation of the susceptibility towards adeno-tonsillar hypertrophy development. PMID: 29501294
- Two DEFB1 SNPs were significantly associated with the DMFT(decayed, missing, filled teeth) index. PMID: 27846636
- Multivariate logistic regression analysis showed that hBD-1 basal cell loss (>/=20% of prostatic glands in total cores) is an independent factor for predicting prostatic adenocarcinoma (odds ratio: 4.739, confidence interval: 1.093-20.554, p = 0.038). hBD-1 loss of basal cells is a useful indicator to identify extremely high-risk patients with initially negative biopsy. PMID: 28885732
- It was concluded that bile acids can differentially regulate colonic epithelial beta-defensin expression and secretion; the authors discuss the implications for intestinal health and disease.- PMID: 28487283
- data provide important insight into the in vivo kinetics of HBD expression, the mechanism of HBD1 induction by HIV-1, and the role of HBDs in the early innate response to HIV-1 during acute infection PMID: 28253319
- CpG methylation frequencies in the DEFB1 low CpG-content promoter were significantly higher in the prostate malignant tissues than in adjacent benign tissues. PMID: 27835705
- DEFB1 - genotypes were correlated with the hBD-1 salivary concentration. PMID: 27770642
- Coding missense and nonsense single nucleotide polymorphisms result in less active HBD1 antibacterial activity against Escherichia coli. PMID: 27160989
- A significant difference was found between the levels of staining of TLR-2, TLR-4 and hBD-1 in different lesions from the epidermis, inflammation region, dermis and skin appendages (p < 0.05) in patients with acne vulgaris. PMID: 26695933
- Expression of hBD-1, hBD-2, hBD-3, and hBD-4 in healthy and chronic periodontitis gingiva. PMID: 26874342
- The -44 CC genotype of the beta-defensin-1 gene (DEFB1 rs1800972) may be associated with susceptibility to chronic periodontitis in Japanese. PMID: 24276427
- No clear association was found between the DEFB1 polymorphisms and tuberculosis. PMID: 26991287
- DEFB1 polymorphisms alone may not influence pathology susceptibility, however they could possibly concur, together with other factors involved in the genetic control of innate immune system, in the predisposition towards recurrent tonsillitis PMID: 26968045
- beta-defensin-1 production is dysregulated in the epithelium of patients with chronic obstructive pulmonary disease PMID: 26568662
- dental plaque amount, lactobacilli count, age, and saliva buffer capacity, as well as DEFB1 gene polymorphism, explained a total of 87.8% of variations in DMFT scores. PMID: 26377569
- Our meta-analysis provides evidence that DEFB1 genetic polymorphisms rs11362G>A, rs1800972C>G and rs1799946G>A are important contributing factors to the development of digestive diseases PMID: 26232989
- Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and TLR2 Arg753Gln and Arg677Trp genes was determined. PMID: 27029120
- Genotypes and alleles of the DEFB1 gene found in long-living individuals can be considered as the factors that increase the probability of longevity and favorable course of age-related diseases. PMID: 26028230
- there is a significant link between innate immunity deregulation through disruption of cationic peptides (hBDs) and the potential development of colon cancer. PMID: 26038828
- Possible association between DEFB1 polymorphisms and in vivo salivary levels of hBD-1 in an Italian population of 40 healthy individuals, finding a genotype-related different expression with two of these polymorphisms: -52G > A and -44C > G. PMID: 25939140
- We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID: 25503380
- A-dependent DEFB1 upregulation below 20 mM predicts in vitro antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation. PMID: 25815330
- We conclude that plant-made recombinant beta-defensins (phBD-1 and phBD-2) are promising antimicrobial substances and have the potential to become additional tools against salmonellosis, particularly when used in combination. PMID: 25417183
- The DEFB1 c.-44C>G polymorphism of the GG protective genotype was much less frequent among multiple sclerosis patients than among the controls. PMID: 26434201
- beta defensin 1 gene -20G/A, -44C/G and -52G/A single nucleotide polymorphisms were not associated with chronic tonsillitis. PMID: 25683590
- Our results suggest the involvement of DEFB1 polymorphisms, in particular that of the c.5*G>A SNP, in the susceptibility of women from Northeast Brazil to HPV infection. PMID: 25410996
- Functional polymorphisms in IL1B and DEFB1 influence susceptibility to mold infection in solid-organ transplant recipients. PMID: 25398456
- DEFB1 minor haplotypes were also associated with an increased risk of developing Atypical Squamous Cells of Undetermined Significance lesions, being significantly more frequent in HPV negative women with lesions, than without lesions. PMID: 24435641
- The present finding explains a common defect in male infertility associated with both asthenozoospermia and leukocytospermia, indicating a dual role of DEFB1 in defending male fertility. PMID: 25122636
- GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin beta-1 production in diabetes. PMID: 25083086
- These findings associate DEFB1 5'UTR polymorphisms with HIV-1/AIDS in Mexican women for the first time. PMID: 25001249
- hBD-1 suppresses tumor migration and invasion of oral squamous cell carcinoma. PMID: 24658581
- Data indicate that CCL2 and IL-10 released from burn patient lineage-CD34+CD31- cells were shown to be responsible for their inhibitory activities on the production of beta-defensin 1 (HBD-1) by epidermal keratinocytes. PMID: 24498256
- A possible involvement of DEFB1 gene in inflammatory bowel diseases. PMID: 24034632
- genetic variant rs11362 in DEFB 1 influences caries susceptibility in CL/P children. The results support the hypothesis that expression of DEFB 1 in saliva may serve as a biomarker for future caries risk PMID: 23964634
- This gene encodes a peptide with antimicrobial activity against S. aureus and H. influenzae. PMID: 20100591
- HBD-1 serum levels in chronic kidney disease patients are inversely related to estimated glomerular filtration rate. PMID: 24356550
- Our findings suggest interplay between hBD-1 and neuroimmunological responses in Alzheimer's disease PMID: 24139179
- IRF5, but not TLR4, DEFEB1, or VDR, is associated with the risk of ulcerative colitis in a Han Chinese population. PMID: 23971939
- Data suggest that levels of DEFB1 and DEFB2 are highest in colostrum and decrease in human milk over course of lactation; abundance of DEFB1 in colostrum may have protective against upper respiratory infection in infants up to 6 months. PMID: 24124699
- The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients, the concentration was significantly higher before treatment than after treatment. PMID: 24564045
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亞細胞定位：
Secreted. Membrane.
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蛋白家族：
Beta-defensin family
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組織特異性：
Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).
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