Human terminal complement complex SC5b9 ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人補體激活產物SC5b9酶聯免疫試劑盒（CSB-E12742h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SC5b9 complex含量。SC5b9 complex即膜攻擊復合體，是補體激活終末途徑形成的產物。其插入細胞膜后可形成跨膜通道，破壞細胞膜完整性，使細胞溶解死亡。當下圍繞它在疾病發生發展機制、藥物靶點等方面的研究較多，為疾病治療提供新思路。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，適用于基礎醫學研究中補體系統激活程度的動態監測，如心血管疾病、腎臟損傷、敗血癥等模型的機制研究，也可用于體外藥物篩選實驗中補體抑制劑或調節劑的效應評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

SC5b9 complex
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 ng/mL-2000 ng/mL
靈敏度：

7.81 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SC5b9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	85-100
1:2	Average %	98
Range %	95-106
1:4	Average %	93
Range %	80-105
1:8	Average %	85
Range %	80-89

回收率：

The recovery of human SC5b9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	89-105
EDTA plasma (n=4)	85	80-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.