Human peroxiredoxin 2 (PRDX2) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人過氧化物酶2(PRDX2)酶聯免疫試劑盒（CSB-EL018654HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PRDX2含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于基礎研究中PRDX2表達水平的動態監測、疾病模型氧化損傷評估、藥物干預效果分析及信號通路機制探索等方向，為細胞實驗、動物模型及臨床前研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PRDX2
Uniprot No.：

P32119
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-95
1:2	Average %	97
	Range %	91-105
1:4	Average %	96
	Range %	92-108
1:8	Average %	93
	Range %	86-102

回收率：

The recovery of human PRDX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	97	92-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
亞細胞定位：

Cytoplasm.
蛋白家族：

Peroxiredoxin family, AhpC/Prx1 subfamily
數據庫鏈接：

HGNC: 9353

OMIM: 600538

KEGG: hsa:7001

STRING: 9606.ENSP00000301522

UniGene: Hs.432121