Human melanin ELISA kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人黑色素酶聯免疫試劑盒（CSB-E14051h）為競爭法ELISA試劑盒，定量檢測血清樣本中的melanin含量。Melanin即黑色素，是一種廣泛存在于人和動物體內的生物色素。其在皮膚顏色形成、抵御紫外線等方面發揮重要作用。研究機制上，主要聚焦于其合成途徑、調控因素等，旨在了解相關機制以開發美白、治療色素異常疾病等方面的方法。試劑盒檢測范圍為12.5 pg/mL-1000 pg/mL，適用于皮膚生理機制、色素代謝調控及黑色素相關疾病模型的科學研究；適用于人體血清中黑色素水平的動態監測；可支持黑色素生成調控機制、光老化研究、美白成分篩選等基礎科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

melanin
種屬：

Homo sapiens (Human)
樣本類型：

serum
檢測范圍：

12.5 pg/mL-1000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-102
1:2	Average %	95
	Range %	91-100
1:4	Average %	94
	Range %	92-97
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.