Human activated protein C,APC ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人活化蛋白C(APC)酶聯免疫試劑盒（CSB-E09909h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APC含量。APC（腺瘤性結腸息肉病基因）是一種腫瘤抑制蛋白，作為Wnt信號傳導途徑的拮抗劑，其突變與家族性腺瘤性息肉病（FAP）和結直腸癌的發生密切相關。研究APC機制主要關注其如何通過調節Wnt通路、細胞遷移、粘附、轉錄激活和細胞凋亡等過程，影響腫瘤的發生和發展。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于凝血調控機制、炎癥相關疾病模型或藥物干預等基礎研究；在血栓性疾病、敗血癥及腫瘤微環境等研究領域具有重要價值本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

APC
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-96
1:200	Average %	94
	Range %	90-97
1:400	Average %	97
	Range %	94-103
1:800	Average %	94
	Range %	90-98

回收率：

The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	87	84-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.