Human activated coagulation factor X(FXa) ELISA kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人活化凝血因子X(FXa)酶聯(lián)免疫試劑盒（CSB-E12696h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FXa含量。FXa（因子 Xa）是凝血級聯(lián)反應(yīng)中的關(guān)鍵酶。其背景在于在凝血過程里發(fā)揮核心作用，參與血栓形成。研究機制方面，聚焦于研發(fā)針對 FXa 的抑制劑，通過阻斷其活性來抑制凝血，進而用于預(yù)防和治療血栓相關(guān)疾病，降低血栓風(fēng)險。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品適用于凝血相關(guān)分子機制研究、血栓性疾病模型分析、抗凝劑藥效評估等科研領(lǐng)域，實驗數(shù)據(jù)穩(wěn)定可靠，配套提供標(biāo)準(zhǔn)品、試劑及操作指南，滿足體外實驗需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FXa
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	92
	Range %	87-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	96	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.