Human Microfibrillar-associated protein 5(MFAP5) ELISA kit
-
中文名稱：人微纖絲關聯蛋白5(MFAP5)酶聯免疫試劑盒
-
貨號：CSB-EL013750HU
-
規格：96T/48T
-
價格：￥3600/￥2500
-
其他：
產品詳情
-
產品描述：人微纖絲關聯蛋白5(MFAP5)酶聯免疫試劑盒（CSB-EL013750HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MFAP5含量。MFAP5 即微原纖維相關蛋白 5，屬于微原纖維相關蛋白家族成員。其與彈性纖維形成、細胞黏附等過程相關。研究機制方面，它在多種疾病如腫瘤、纖維化疾病中異常表達，可能通過影響細胞外基質等發揮作用，是潛在的疾病治療靶點。試劑盒檢測范圍為12.5pg/ml- 800pg/ml，適用于心血管疾病機制研究、腫瘤微環境分析、組織再生能力評估等科研領域，適用于體外研究場景，可為探索MFAP5在細胞外基質重塑、疾病生物標志物篩選等方向提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：AAT9 ELISA Kit; MAGP 2 ELISA Kit; MAGP-2 ELISA Kit; MAGP2 ELISA Kit; MFAP 5 ELISA Kit; MFAP-5 ELISA Kit; MFAP5 ELISA Kit; MFAP5_HUMAN ELISA Kit; Microfibril associated glycoprotein 2 ELISA Kit; Microfibril-associated glycoprotein 2 ELISA Kit; microfibrillar associated protein 5 ELISA Kit; Microfibrillar associated protein 5 precursor ELISA Kit; Microfibrillar-associated protein 5 ELISA Kit; MP25 ELISA Kit
-
縮寫：
-
Uniprot No.：
-
種屬：Homo sapiens (Human)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測范圍：12.5pg/ml - 800pg/ml
-
靈敏度：3.125pg/ml
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Others
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
-
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MFAP5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
97
Range %
92-103
1:2
Average %
91
Range %
82-97
1:4
Average %
103
Range %
99-109
1:8
Average %
87
Range %
82-97
-
回收率：
The recovery of human MFAP5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
90
85-99
EDTA plasma (n=4)
111
105-116
-
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
800
2.591
2.612
2.602
2.461
400
2.247
2.251
2.249
2.108
200
1.630
1.655
1.643
1.502
100
1.085
1.074
1.080
0.939
50
0.654
0.698
0.676
0.535
25
0.396
0.401
0.399
0.258
12.5
0.263
0.245
0.254
0.113
0
0.144
0.137
0.141
-
數據處理：
-
貨期：3-5 working days
產品評價
相關產品
靶點詳情
-
功能：May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity. Component of the elastin-associated microfibrils.
-
基因功能參考文獻：
- MFAP5 was up-regulated in breast cancers compared with that in normal breast tissues, and further increased in breast cancer bone metastasis. Functionally, MFAP5 overexpression accelerated breast cancer cell proliferation and migration, while an opposite effect was observed when MFAP5 was knocked down. PMID: 29526753
- Study shows that over-expression of MFAP5 and TNNC1 is correlated with cervical lymph node metastasis (CLNM), metastasis relapse-free survival and overall survival. These results propose that MFAP5 and TNNC1 may be potential markers for predicting occult cervical lymphatic metastasis and prognosis of oral tongue carcinoma. PMID: 27713166
- Likely pathogenic variants included a TGFB2 variant in one patient and a SMAD3 variant in another. These variants have been reported previously in individuals with similar phenotypes. Variants of uncertain significance of particular interest included novel variants in MYLK and MFAP5, which were identified in a third patient PMID: 26854089
- The results answer the question of how MAGP2 controls cell type dependent Notch signaling, but more importantly uncover a new mechanism to understand how extracellular matrices and cellular environments impact Notch signaling. PMID: 26808411
- Our results demonstrate that factors involving low-grade inflammation modulate MFAP5 expression and that the modified expression of MFAP5 may further regulate adipose tissue inflammation. PMID: 26054006
- FAK/CREB/TNNC1 has a role in mediating the effect of stromal MFAP5 on ovarian cancer metastatic potential PMID: 25277212
- Alteration of MAGP-2, a component of microfibrils and elastic fibers, appears as an initiating mechanism of inherited Thoracic aortic aneurysm and dissection (TAAD). PMID: 25434006
- EPS led to the discovery of two novel immunomodulatory proteins, MFAP5 and PENK that when administered to mice subjected to endotoxemic shock, reversed the cytokine storm and provided a significant survival benefit PMID: 24496384
- Decreased MFAP5 gene expression in the endometrium of patients with implantation failure after in vitro ertilization treatment PMID: 22885067
- The MAGP2-based assay provided superior performance for the purpose of cell culture identification compared to assays using standard reference genes. PMID: 20345228
- microfibrillar proteins MAGP-1 and MAGP-2 can function outside of their role in elastic fibers to activate a cellular signaling pathway PMID: 16492672
- MAGP-2 promotes angiogenic cell spouting in vitro by antagonizing Notch signaling pathways in endothelial cells. PMID: 18417156
- Independent evaluation confirmed the association of a prognostic gene microfibril-associated glycoprotein 2 (MAGP2) with poor prognosis in advanced ovarian cancer PMID: 19962670
顯示更多
收起更多
-
相關疾病：Aortic aneurysm, familial thoracic 9 (AAT9)
-
亞細胞定位：Secreted, extracellular space, extracellular matrix.
-
蛋白家族：MFAP family
-
數據庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
