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Human Interferon β,IFN-β/IFNB ELISA Kit

A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IFN-β This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled IFN-βantibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	100
	Range %	91-110
1:4	Average %	97
	Range %	92-105
1:8	Average %	93
	Range %	89-98

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.