Human Enteropeptidase(PRSS7) ELISA kit

Human Enteropeptidase(PRSS7) ELISA kit

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中文名稱：

人腸肽酶(PRSS7)酶聯免疫試劑盒
貨號：

CSB-EL018824HU
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人腸肽酶(PRSS7)酶聯免疫試劑盒（CSB-EL018824HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PRSS7含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品適用于基礎科研中探索PRSS7在胃腸功能調控、營養代謝相關實驗模型中的表達動態，或用于評估特定干預措施對腸道蛋白酶活性的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TMPRSS15; ENTK; PRSS7; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15
縮寫：

PRSS7
Uniprot No.：

P98073
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-110
1:2	Average %	90
	Range %	85-85
1:4	Average %	92
	Range %	88-98
1:8	Average %	98
	Range %	93-104

回收率：

The recovery of human PRSS7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.528	2.422	2.475	2.358
10	1.746	1.692	1.719	1.602
5	1.164	1.110	1.137	1.020
2.5	0.654	0.649	0.652	0.535
1.25	0.413	0.434	0.424	0.307
0.625	0.271	0.253	0.262	0.145
0.312	0.195	0.208	0.202	0.085
0	0.120	0.114	0.117

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
基因功能參考文獻：
1. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 106 M-1 sec-1) and DDDDR (kcat/KM = 1.89 x 107 M-1 sec-1) relative to all other enteropeptidase variants reported to date. PMID: 23436726
2. Enteropeptidase is a gene associated with a starvation human phenotype and a novel target for obesity treatment PMID: 23185382
3. Human enteropeptidase shows 10x faster kinetics compared to other animal sources but low solubility under low salt conditions. A supercharged variant of enteropeptidase light chain with increased solubility was used for crystallization. PMID: 22488687
4. Characterization of the different catalytic activity of human and bovine enteropeptidase light chains toward hydrolysis of peptides and proteins lacking tetraaspartate sequence. PMID: 22571433
5. Engineered recombinant enteropeptidase catalytic subunit: effect of N-terminal modification PMID: 11913964
6. Produced in enterocytes and goblet cells. Localization on brush border of cells for physiological activation of digestive enzymes. In duodenal polyps and adenocarcinoma at duodenum but not in Brunner's gland adenoma. PMID: 12907431
7. Enterokinase directly cleaved proMMP-9 at the Lys65-Ser66 site. PMID: 18062964
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相關疾病：

Enterokinase deficiency (ENTKD)
亞細胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Peptidase S1 family
組織特異性：

Intestinal brush border.
數據庫鏈接：

HGNC: 9490

OMIM: 226200

KEGG: hsa:5651

STRING: 9606.ENSP00000284885

UniGene: Hs.149473

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.