Human Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Ⅰ型前膠原羧基端肽(PⅠCP)酶聯免疫試劑盒（CSB-E08079h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的PⅠCP含量。人Ⅰ型前膠原羧基端肽（PⅠCP）是反映骨代謝的重要指標，其水平變化與骨吸收和骨形成密切相關。研究顯示，PⅠCP在骨質疏松、骨折愈合等疾病診斷和治療監測中具有重要作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，常用于骨轉化研究、纖維化疾病模型評估及組織修復機制探索等科研領域。適用于骨代謝相關細胞實驗、動物模型研究，以及肝纖維化、肺纖維化等疾病機制研究，尿液樣本的兼容性為無創性研究提供了便利。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
縮寫：

PⅠCP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	99-101
1:200	Average %	91
	Range %	85-97
1:400	Average %	87
	Range %	82-94
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of human PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-93
EDTA plasma (n=4)	100	93-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.