Human Bladder tumor antigen,BTA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人膀胱腫瘤抗原(BTA)酶聯(lián)免疫試劑盒（CSB-E08926h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BTA含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，科研領(lǐng)域可用于膀胱癌發(fā)病機(jī)制研究、抗腫瘤藥物作用效果評估、腫瘤動物模型體液標(biāo)志物檢測等方向，亦可結(jié)合組織病理學(xué)分析探索BTA在腫瘤微環(huán)境中的動態(tài)變化規(guī)律，為生物標(biāo)志物篩選和分子診斷技術(shù)開發(fā)提供實(shí)驗(yàn)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

BTA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BTA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-99
1:2	Average %	102
	Range %	95-105
1:4	Average %	95
	Range %	86-98
1:8	Average %	97
	Range %	91-102

回收率：

The recovery of human BTA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-97
EDTA plasma (n=4)	93	89-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.