Horse insulin-like growth factor 1 (IGF1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

馬胰島素樣生長因子1(IGF1)酶聯免疫試劑盒（CSB-EL011086HO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF1含量。IGF1即胰島素樣生長因子 1，在人體生長發育、細胞增殖分化等生理過程中發揮重要作用。其異常表達與癌癥、糖尿病等疾病密切相關。研究機制多聚焦于它與受體結合激活下游信號通路，以探索通過干預該靶點治療相關疾病的策略。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于馬屬動物相關的基礎研究，如運動生理學分析、生長調控機制探索、代謝性疾病模型構建等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit; Fragment ELISA Kit
縮寫：

IGF1
Uniprot No.：

P51458
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	95
	Range %	90-104
1:4	Average %	92
	Range %	87-100
1:8	Average %	89
	Range %	80-97

回收率：

The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-101
EDTA plasma (n=4)	87	82-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
亞細胞定位：

Secreted.
蛋白家族：

Insulin family
數據庫鏈接：

STRING: 9796.ENSECAP00000008158

UniGene: Eca.12985