Hamster Interleukin 1β,IL-1β ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

倉鼠白介素1β (IL-1β)酶聯免疫試劑盒（CSB-E14259Ha）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL1B含量。IL1B 即白介素-1β，是一種促炎細胞因子。它在炎癥反應、免疫調節等生理和病理過程中扮演重要角色。研究表明，IL1B 可激活下游信號通路，促進炎癥因子釋放，與多種炎癥性疾病、自身免疫性疾病的發生發展密切相關，是潛在的治療靶點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外炎癥模型構建、免疫調控機制研究以及生物制劑或天然產物的抗炎效果評價等科研場景，為探索IL - 1β在倉鼠疾病模型中的生物學功能提供可靠工具。其IL - 1β動態變化常用于評估感染性疾病模型、自身免疫機制及炎癥相關藥物干預研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-1β
Uniprot No.：

Q9WVQ9
種屬：

Mesocricetus auratus (Golden hamster)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	105
	Range %	100-112
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of hamster IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.