Grouper Vitellogenin,VTG ELISA Kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

石斑魚卵黃蛋白原(VTG)酶聯免疫試劑盒（CSB-E14116Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VTG含量。試劑盒檢測范圍為50 ng/mL-2000 ng/mL，適用于水產養殖中魚類繁殖力評估、環境污染對生殖系統影響的機制研究，以及水生態毒理學領域的生物監測實驗。為海洋生物生殖發育研究及生態毒理評估提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
縮寫：

VTG
Uniprot No.：

Q5IGP8
種屬：

Grouper
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

50 ng/mL-2000 ng/mL
靈敏度：

50 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	97
	Range %	91-102
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	95	91-101