Duck Interferon γ,IFN-γ ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

鴨γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E08902Du）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFN-含量。IFN-（干擾素相關靶點）在免疫調節中意義重大。其背景在于它與機體抗病毒、抗腫瘤等免疫反應密切相關。研究機制方面，主要是通過與細胞表面受體結合，激活相關信號通路，誘導多種抗病毒、免疫調節蛋白的表達，以發揮生物學效應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研場景中鴨類病毒感染模型研究、疫苗免疫效果評價、免疫調節分子通路探索等方向本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫：

IFN-
Uniprot No.：

Q9YGB9
種屬：

Duck
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of duck IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	98
	Range %	92-106
1:4	Average %	85
	Range %	81-86
1:8	Average %	88
	Range %	83-94

回收率：

The recovery of duck IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	103	101-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
亞細胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
數據庫鏈接：

KEGG: apla:101790439